We narrowed to 402 results for: LARGE

1. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   Published

   April 13, 2023, 1:15 p.m.

   ...viability. Library delivery Screening libraries, large collections of biological independent variables,...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives...

3. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...themselves using computational approaches to analyze large data sets (myself included) and we are faced with...

4. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...re happy to provide them, that never works on a large scale. The world is too big now. Mendenhall: So ...

5. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

6. CRISPR/Cas9 FAQs Answered!

   Type

   Blog Post

   Published

   March 13, 2014, 4:08 p.m.

   ...with the Cas9/sgRNAs vector (like PX330). For large changes (>100 bp insertions or deletions), we typically...lab typically use PAGE purified long oligos. For large changes (>100 bp insertions or deletions), the Zhang...

7. Supporting Rare Disease Research with the 2020 BeHEARD Awards

   Type

   Blog Post

   Published

   Sept. 8, 2020, 2 p.m.

   ...in this educational video. “SLC6A8 belongs to a large family of solute channels that are responsible for...

8. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...many samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An ...

9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...microscope showed us that, in monkey neurons, the large majority of the gold particles were in the cytoplasm...after it has been administered. Although we found a larger proportion on the membrane in mice neurons, most...

10. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...used in experiments. Retroviral vectors make up a large toolbox used by researchers for (among other things...

11. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...germline editing. Although this paper represents a large step forward in the CRISPR field, it’s important...

12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ... can be excited many times, thereby producing a large number of emitted photons before they are destroyed...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...to be large enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain...lentiviral vectors are superior packaging vectors for larger genes - they can hold up to 8 kb, while AAV can...

14. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...) and even on 60 Minutes. We are blessed with a large community of scientists who support us by depositing...

15. Click Biology: How to Click and Play to Explore Biology

    Type

    Blog Post

    Published

    Oct. 21, 2025, 1:15 p.m.

    ...-assisted insertion tagging Kit; integration of large DNA fragments at user-defined locations NEB Cell-Imaging...

16. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ..., two full antibodies bound together may be too large. Using a complex that is one full antibody bound...and have lower binding affinity than some of the larger fragments.  Think of antibody fragments as new ...

17. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...communication has helped Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 ...

18. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ... lab management software aren’t just limited to large volume facilities; it can be useful in academic ...

19. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...CRISPR guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-...

20. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...