...less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing...associated with gRNA introduction (Allen, et al., Frontiers in Genome Editing). This is particularly important...CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https://doi.org/...

...lab mates are coming and going frequently - an environment ripe for valuable work and materials to slip ...think it's worth consideration. Everything will go wrong Whenever an order is placed on the Addgene website...which part of her procedures are the most failure prone. In an academic lab, a similar situation could arise...

... these conditions. On top of these factors, environmental conditions such as cellular redox state and ...et al, 2012). The third issue relates to the environment of the fluorescent protein. Photostability measurements...in vitro methods allow for a well-controlled environment, but they do not mimic the natural situation....

... of a toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during...cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin protein (CcdA) that protects... 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989 May;171(5):2353...

...us, as much as it as about the amazing years in front of us. So in our last celebratory moment, we wanted...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...

...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...

...Methods 2018. PubMed PMID: 30377362. Studying neuronal activity using the fluorescent voltage indicator...Listen to the voltage indicator podcast segment The neurons in the brain compute using electrical impulses ... cloning system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently...

...taken in an industry, regulatory, or clinical environment. It remains contested, however, whether a better...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...

...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...online content for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs...Plan Learn a Little about The Academic Labor Environment from the Future of Research Symposium Resources...

... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316... Michelle Cronin...

... affinity terminator may be desired for multi-cistronic constructs where high termination efficiency is...Bacterial Transcription Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297...Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional...

...fluorescent proteins, has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike ...and acceptor is sensitive to a change in the environment which alters the FRET efficiency. Detecting certain...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find...

... challenge with using AdV is that it triggers a strong immune response after in vivo administration, which...loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal...identified, re-transform into a bacterial strain not prone to recombination for amplification. The final purified...

... precision medicine research. A willingness to confront these tough issues is essential for building a...careers should take advantage of their academic environment and attend seminars, symposia, or courses in ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... some in-depth grant writing tips at the Human Frontier Science Program's website. One way to add originality...get some critical insight into what you're doing wrong. All this helps to improve your application for ...

...Compensation beads are a convenient tool to obtain a strong positive signal for a given fluorophore, as they...won’t bind anything (negative population). It is strongly recommended that when you first run a new panel...compensation beads are convenient in that they provide a strong positive signal, even for rare cellular markers...

...possible because baculoviruses are slightly less prone to recombination than other viruses, like lentivirus.... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https:...

...genomic site can be edited based on its chromatin environment. After selecting the best pegRNA design with ...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid...

...from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT...