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We narrowed to 580 results for: RON

Showing: 201 - 220 of 580 results

  1. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...PMC9015817.   Meet anti-GFP [N86/8R]! by: Ashley Waldron Anti-GFP [N86/38.1R] was among the first batch ...microscopy applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly...expansion microscopy as well as correlative light and electron microscopy and observed high fluorescence resilience...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

  3. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...to graduate from university, many students are confronted with the question ‘what now?’ This is often a...good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...exercise these skills outside of a formal work environment also shows that you are passionate and capable...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ..., which uses formaldehyde or UV light to create strong but reversible bonds. This will keep the protein...of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary. This....com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...to create a more productive and cohesive team environment. Neglecting communication skillsClear communication... great "baby project," offering a supportive environment to build my management skills. Finally, developing...effectively and foster a cohesive, productive environment. For example, in my current role managing a cross-functional...

  6. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...scientific community.Paratore: I’ve certainly had a front row seat watching the international growth through...whether that be academically or on the employment front. Paratore: I work at Tufts Health Plan and my role...operations. We’re looking for those “what could go wrong scenarios” and making sure we have adequate process...

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing...associated with gRNA introduction (Allen, et al., Frontiers in Genome Editing). This is particularly important...CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https://doi.org/...

  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...

  9. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...Methods 2018. PubMed PMID: 30377362. Studying neuronal activity using the fluorescent voltage indicator...Listen to the voltage indicator podcast segment The neurons in the brain compute using electrical impulses ... cloning system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...us, as much as it as about the amazing years in front of us. So in our last celebratory moment, we wanted...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...

  11. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... these conditions. On top of these factors, environmental conditions such as cellular redox state and ...et al, 2012). The third issue relates to the environment of the fluorescent protein. Photostability measurements...in vitro methods allow for a well-controlled environment, but they do not mimic the natural situation....

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ... of a toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during...cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin protein (CcdA) that protects... 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989 May;171(5):2353...

  13. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...lab mates are coming and going frequently - an environment ripe for valuable work and materials to slip ...think it's worth consideration. Everything will go wrong Whenever an order is placed on the Addgene website...which part of her procedures are the most failure prone. In an academic lab, a similar situation could arise...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...taken in an industry, regulatory, or clinical environment. It remains contested, however, whether a better...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...

  15. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...online content for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs...Plan Learn a Little about The Academic Labor Environment from the Future of Research Symposium Resources...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... precision medicine research. A willingness to confront these tough issues is essential for building a...careers should take advantage of their academic environment and attend seminars, symposia, or courses in ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

  18. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... challenge with using AdV is that it triggers a strong immune response after in vivo administration, which...loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal...identified, re-transform into a bacterial strain not prone to recombination for amplification. The final purified...

  19. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...fluorescent proteins, has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike ...and acceptor is sensitive to a change in the environment which alters the FRET efficiency. Detecting certain...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... some in-depth grant writing tips at the Human Frontier Science Program's website. One way to add originality...get some critical insight into what you're doing wrong. All this helps to improve your application for ...
Showing: 201 - 220 of 580 results