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We narrowed to 749 results for: SOM

Showing: 201 - 220 of 749 results

  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP,...biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves...filters (e.g. GFP also emits yellow light). Note that some combinations of FPs can cause an effect called FRET...brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657, which has...which is slightly acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...technology and its potential for the treatment of some genetic diseases. I didn’t know it at the time..., including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset form of blindness...expression in non-dividing cells because they remain in episomal form in the cell’s nucleus. But since nothing...age-related macular degeneration are ongoing and some will hopefully follow in the footsteps of Luxturna...despite promising results in preclinical studies some potential candidates for rare to ultra-rare ocular...

  3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...technology. Can you describe this technology and some of its potential applications? Fujii: We generated...amplification of specific DNA sequences. Thus, we've made some contributions in technology development in biology...different experiences in research labs. What are some similarities and differences you have observed as...publications in a short period of time and may need to do some fashionable projects. However, I hope that they ... they don't stop, they may be able to achieve something that may change the rules of the game. In this...

  4. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    Published
    Nov. 30, 2015, 3:30 p.m.
    ...but his short arms are causing him some difficulty… To give him some inspiration, we’d love to see how ... upcoming wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates...will warm up the entire lab (hint: you might need some mini marshmallows…). We’ll choose 4 submissions ...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...genetic similarities with humans. However, rats have some key differences in physiology, intelligence, and...science with early research providing insight into some of the most significant and fundamental biological...in vivo studies. These attributes make frog eggs some of the most useful tools in developmental and biomedical...commonly used species of frog. (Liu et al., 2016). Some of the most popular tools for studying frogs include...world worked to characterize each gene’s function. Some key genetics research with Arabidopsis include understanding...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...While many transgene will fit within this limit, some like prime editing's PE2 enzyme do not. So how do...is the key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve...Chamberlain et al., 2016). It’s generally accepted that some form of homologous recombination (HR) occurs via....org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi... Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M,...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...different CIB constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...A) OptoPLD is recruited to plasma membrane or lysosomes and its activity can be monitored by phosphatidic...enzymes. Conclusion We hope this post has illuminated some of the many tools available for controlling cell...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...) Micrograph of H1.8-GFP nucleosomes collected with MagIC-cryo-EM. Nucleosome-like particles identified...Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository... the obtained structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under...resolution) of the linker histone H1.8 in Xenopus nucleosomes and in complex with its chaperone NPM2, but the...

  9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...While many labs approach the situation differently, some adopting simple note-taking tools, others using ...including reports and protocols. To facilitate this, some ELNs allow you to invite people and assign them ...records on who did what and when) important to you? Some ELNs record the times and dates of your actions....team is. Once you’ve made your list of ELNs, create some free or demo accounts and schedule online meetings...data is organized within an ELN already. Investing some time in deploying a software solution that makes...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... dyes usually spread unidirectionally, there are some reports of bi-directionality. For predominately ...been modified and used as tools for tracing. With some engineering, RABV and HSV, but also others including...vectors have been widely used by neuroscientists for some time due to their advantageous characteristics for...retrograde and anterograde intracellular transport of some AAV serotypes, but overall with rather low efficiency...however, a few reports of trans-synaptic spreading of some AAVs, specifically AAV6 in the retrograde direction...monosynaptic and polysynaptic spreading. AAV vectors cover some of these attributes and their use has allowed for...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...c-MYC is the established model for reprogramming somatic cells. A common method for generating iPSCs to ...-excitable organge fluorescent protein (CyOFP1), some of the exciting properties of mCyRFP1 include a ...with HDR. Coselection efficiency is so high that some applications may permit working with edited “pools...see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are comprised of 2 plasmids, pPSU1...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops... themselves against bacteriophage infection, but some phages fight back using CRISPR inhibitors. Several...

  12. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP), or lysosomal-associated membrane...Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository...imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise...(PV) immunostaining (green), in the primary somatosensory cortex (S1) of the mouse brain following retro-orbital...

  13. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    Published
    Aug. 17, 2021, 1:15 p.m.
    ...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...level up in the lab, and we’ve started collecting some of our favorite (moving) images on a GIPHY page!...so well you need to express yourself. We have something for that too.   This could be you after...emotions (while you’re on a break from expressing some genes of interest).   Blugene is so excited...

  14. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... appropriately validated? This post will go over some of the general considerations you should review ...you may need to roll up your sleeves and perform some extra validation experiments yourself.  Goal of ...be concerned that your antibody is binding to something unexpected. This approach has high specificity...sites or alternative splicing that could lead to some form of the target protein to be present and recognizable...can be difficult to find epitope information for some antibodies, which makes it hard to ensure that you...

  15. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...students split into two groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids...our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to learn about intellectual... science and business or programming were new to some, who were surprised at “how much business there ...researchers, so Outreach Scientist Jessica Welch showed some highlights of his travels from 2015, including a...

  16. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...career paths. Check out Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...Flow cytometers however are highly versatile and some can accommodate up to 30 different colors. This ...the tag can alter the structure of the protein. In some cases this can lead to changes or a complete loss... lipids including those of the cell membrane. In some cases, this can mask epitopes such that immune complexes... complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...overview of flow cytometry for single cell analysis and some useful background information for setting up antibody...

  18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...this blog is here to help. Here, we will outline some fundamental characteristics to consider when selecting...make your selection easier, as well as highlight some recent advancements within this field. Fundamental...be covalently attached to self-labeling proteins. Some common examples of these self-labeling proteins ... and its propensity to bind cellular components. Some dyes target specific organelles like mitochondria...technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to the permeation...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ... their work. Thousands of people showed up; some with some really great signs. The Congressman for Albany...Harvard Medical School working on human artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa...lab where the groundbreaking research happens, sometimes forgetting how to engage with friends, family,...science professionals and supporters. I'm raising some little scientists (as you can see in the picture...

  20. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...project. You’ve met someone whose career interests you or you want to pursue someone doing a job you wish...potential to alienate connections. If meeting with someone to ask about a job or a particular position at ...interest in a job. Ask openly to be connected to someone who can help you land that position such as a direct...
Showing: 201 - 220 of 749 results