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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 281 results for: hal.1

Showing: 201 - 220 of 281 results

  1. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

  2. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...products by their environmental impact on a scale of 1-10: the lower the score, the lower the environmental...wash your tips once and you cut your tips cost in half, not to mention the amount of plastic you’ve saved...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...the output of the analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...together along with the Gibson assembly master mix for 1 hour, and then transform into competent cells. The...you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...

  6. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...compare the experimental group.     Figure 1: A, Most biological experiments will benefit from ... Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications, Jan. 2011, pp. 6–12. Crossref,...possible due to a lack of available materials, lethality of knockdown, or other factors, consider using...

  7. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    .... Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research...since the Future of Research Symposia Ongoing challenges facing the scientific enterprise and the way ...landscape of the scientific enterprise will provide challenges and opportunities for us all.  For example, implementation...categorize all postdocs at institutions, will be challenging. At the same time, there is much opportunity ...the pressure on for further increases will be challenging, but will be aided by the development and implementation... is an important way to push the balance from challenge to opportunity. We hope to see you at the upcoming...

  8. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image used...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...

  9. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...in restraint. The very first subheading in Chapter 1 asks “What is science?” That’s a very big question...funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly politicized...., “The Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other...ramifications. Readers unfamiliar with academia’s challenges with publication and funding may be dismayed ...The three chapters in this section describe the challenges of trusting science in a “fake news and post-...describes the author’s thoughts on science’s biggest challenges over the coming years. It is arguably the most...the book? It doesn’t need to. The problems and challenges described in The State of Science have not changed...

  10. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are happy...Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes, work...and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are not solely...absolutely equally, which means you only have one-half of the guilt. You can handle that, right? Big tip...get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy...yourself; you will make progress over time. #6 Thou shalt throw money at the problem If you can, pay someone...to relax and be with friends and family. #7 Thou shalt create community to help Involve family. If you ...

  11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics...can be simply introduced by injection. The main challenge in using ligands is ensuring the agonist is able...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ... email in a busy inbox. Updated policy As of July 1, 2025, we are updating our Hold for Publication policy...choose this status for your plasmids. In fact, almost half of all new deposits start out with this hold! You...

  13. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system...traditional FP fusions can also affect protein activity, half-life, or localization, these concerns are greater...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ...split you got from the graduate student down the hall who got it from some technician downstairs who got...

  15. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ... enzymes target several strains of E. coli and lethally chop their genomes into many, many pieces. Locus...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... end sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon...proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas systems, in contrast to single-protein...with the transposition protein TniQ for targeting (Halpin-Healy et al., 2020). Genomic targets require an...creates a 5-bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons...follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS...doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH (2019) Transposon-encoded...

  17. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity...especially in situations when it is technically challenging to collect large numbers of cells or when a high...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...updated version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation...constructs, localization of the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering...

  19. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...them you can do today! 10 ways to share your science 1)   If you don’t already make it a habit to talk – ...   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding...

  20. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...then scroll on for some wintery delights!    Table 1: New ready-to-use viral vectors in the Addgene repository...Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep ...
Showing: 201 - 220 of 281 results