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We narrowed to 363 results for: his

Showing: 201 - 220 of 363 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...field in which covalent modifications to DNA or histones, the proteins that help package DNA, were associated...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification...researchers additionally fused a TAL effector to LSD1 histone demethylase to demethylate enhancer regions (Mendenhall...regulate transcription, also includes light-regulated histone methyltransferases and deacetylases. CRISPR and... acetyltransferase increases levels of H3K27ac histone modification at specified loci. Charles Gersbach... Figure 1: dCas9-p300 adds H3K27ac marks to histones in promoter and enhancer regions. These marks ... for targeted removal of H3K4me1/2 and H3K9me2 histone modifications. Like the TALE-LSD1 system described...

  2. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E HIST1H2BB HIST2H2BE RBX1 STAG2 Xist Your favorite gene...context of chromatin?” Though it’s true that adding histones and chromatin architecture to every diagram portraying...transcription of a gene a megabase away, or looking at the histone methylation status at a double-strand break that...2018 Keystone meeting for Chromatin. Note that histone genes frequently have many different variant names...RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl ...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... also be used to engineer new pathways with sophisticated gene expression controls. Most cell types do...two-component systems (TCSs). TCSs consist of a histidine kinase that phosphorylates a response regulator...domain from cyanobacteria to a common E. coli histidine kinase. This hybrid construct senses light, and...light; these two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...light-sensitive two-component system (TCS). A hybrid histidine kinase/photosensor senses various light inputs...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody....1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian O’Neill The Cohen...created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion protein...ticker tape images could recover transcriptional histories, accurate to within 40 minutes. Figure ...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...structures (at < 5 Å resolution) of the linker histone H1.8 in Xenopus nucleosomes and in complex with...has developed a new epigenetic editor, Coupled Histone tail for Autoinhibition Release of Methyltransferase...can be toxic, CHARM uses a cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase...uses a fusion of the DNMT3L cofactor (D3L) with a histone tail (H3K4me0) to a DNA-binding protein (dCas9 ... a familiar challenge for anyone who has done histology on brain tissue is identifying the cells you are...

  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...situ hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...Cytochemistry : Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional...

  7. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine ... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

  8. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...epitope tag (6xHis or GST) to affinity purify the expressed protein with a specific resin (e.g. 6xHis containing...level expression? Finally, you can add bells and whistles to your construct. If you are planning to track...containing pDEST-HisMBP). After purification the protein can be released from the resin and used for various...

  9. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...skeptical reader of historical fiction (I can personally attest to this!). Following history from the Ice Age...Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling magicians in the...of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length may be intimidating...

  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which SNPs ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...are five more model organisms that have left a historical impact on the scientific community. (And, check...  The E. coli genome has an extensive research history and characterization. Many proteins and processes...organisms in the world. With its extensive scientific history, highly characterized genome, and widespread utilization...organisms is its thoroughly developed and defined history in research (Krämer, 2015). As a result, a vast...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...technological innovations. Let’s take a look at three historical techniques: southern blotting, restriction mapping...throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below!...Benjamin Chain. "The sequence of sequencers: The history of sequencing DNA." Genomics (2015). PubMed PMID...

  13. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...Stephen M., et al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. ...Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications...and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal...

  14. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each...described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1) assessed...

  15. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...a nucleus -> DNA compaction around histones -> Examples of histone-modifying enzymes and reader-effector... specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these modules...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ....8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464 (... 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) 465 (...2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein...

  19. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

  20. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...long, whistling while we work. This is certainly not the truth (partially because I can’t whistle). No,...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...
Showing: 201 - 220 of 363 results