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We narrowed to 813 results for: org

Showing: 201 - 220 of 813 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...

  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ... Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska...and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al ...SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the...Against Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody...

  3. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org/10.1101...microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   Novel class of...Cellular Biology, 5(12), 3610–3616. doi: https://doi.org/10.1128/mcb.5.12.3610-3616.1985.       ...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava...Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman...Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069  Additional resources on ...parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov, ...In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional resources... precise targeted manipulation Resources on Addgene.org Browse all viral vectors available at Addgene...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy...provides limited information about what materials or organelles are being targeted for autophagy. The development...for reporting vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

  8. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran WF, Lam...severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam...humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional resources... Learn more about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection...

  9. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun A (2015)...recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein ND, Thie...functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional resources...recombinant monoclonal antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene...

  10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (...Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW...Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/10.1177/38.7.2355173  Additional resources on the...Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene   ...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict...Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L,...Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources on the...cell-type expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV...

  12. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith O, Tilmans...Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke...Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources...viral vectors protocols and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's...

  13. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working with viruses...please email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated... we distribute, please send an email to help@addgene.org. Your questions help us expand and improve our...Expression in Mammals Additional Resources on Addgene.org Overview of Viral Production at Addgene Find...

  14. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article   ...

  15. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...

  16. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare publications...publications or your dissertation Organize data that you already collected into figures for papers or...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Please send any questions or comments to help@addgene.org. Finally we’d like to thank all those who are...Viral Transductions Additional Resources on Addgene.org Visit the Viral Service Page Check Out Our CRISPR...

  18. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...Develops Lab Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On...

  19. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

  20. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    .... 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038/s41467-...Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered miniature...Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...
Showing: 201 - 220 of 813 results