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  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...then injected into a blastocyst. There are several types of genetically engineered mice: transgenic mice,...in part 2 of this blog series) controls the cell types in which the conditional recombination can occur...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves to promote...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...Prader–Willi syndrome, which have very different disease phenotypes, are two fascinating examples of epigenetics ... chromosome 15 are inactivated, but different phenotypes result depending upon whether the improperly ...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ... Viruses can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms...viruses can be produced in multiple different cell types, including plants, mammals, and insects. In this...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...collect will depend on the serotype, as some rAAV serotypes are abundantly released into the culture media...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...more suited for prediction of very diverse edit types and cell contexts. Figure 1: High-throughput... diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016/j.cell...

  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...control methods and compare the commonly available types of degron tags. What is a degron tag and why should...options! Below we will review the 4 most common types of degrons and some of the nuances to each one. ...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...video and reference on how to design PCR primers.  Types of PCR Since the invention of PCR, different PCR... analysis, and RNA detection.  A summary of the types of PCR   Type of PCR Goal of the PCR method ...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...light does not overlap in wavelength. In many microscopes the filters are not narrow enough to distinguish...Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent ...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...contained a viral envelope to dictate the tropism, or types of cells the vector could infect. Finally, the transfer...envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary cells or...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their phages ...Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria. It’s a...

  14. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...location, location, location There are many different types of samples at Addgene - DNA sent from depositors...barcodes additionally allow us to store multiple types of tube for each plasmid we have at Addgene. Each...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...immunotherapy than ever before. Although different types of cancer have different challenges and obstacles... mesothelioma make it different from many other types of cancer. Mesothelioma is considered an orphan ...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...collaborate whenever it faces an important challenge. The types of projects mentioned above are just examples, and...leverage the preferences of different personality types, and to devise strategies to be prepared for addressing...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...enhanced editing efficiencies in a number of cell types at different genomic loci — in some cases, over ... Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid protein, one of...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...regions. They also don’t give any clues about the types of neurons involved or whether their involvement..., et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute ...
Showing: 201 - 220 of 429 results