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...studies in neuroscience where specific tissues, cell types, or even subcellular regions of a neuron need to...expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase systems...

...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others...addition, scientists have engineered entirely new types of plasmids without clear analogues in nature. For...

...then injected into a blastocyst. There are several types of genetically engineered mice: transgenic mice,...in part 2 of this blog series) controls the cell types in which the conditional recombination can occur...

...a paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following...fields of research they support. Monitoring these types of accounts can help you find papers related to ...

... readers to more information if they desire it. Shapes. In this type of poster, written and graphical ...trickier to make figures fit nicely into circular shapes rather than a square or rectangular boxes. Background...

...turns green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte...well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein ...

...and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves to promote...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal...

...Prader–Willi syndrome, which have very different disease phenotypes, are two fascinating examples of epigenetics ... chromosome 15 are inactivated, but different phenotypes result depending upon whether the improperly ...

...you to understand how to work with many different types of people both above and below you. During my time...of the NPA. You must accommodate many different types of people in order to harness their strengths and...

...video and reference on how to design PCR primers.  Types of PCR Since the invention of PCR, different PCR... analysis, and RNA detection.  A summary of the types of PCR   Type of PCR Goal of the PCR method ...

...control methods and compare the commonly available types of degron tags. What is a degron tag and why should...options! Below we will review the 4 most common types of degrons and some of the nuances to each one. ...

...packaging plasmids for AAV6 and AAV11! Why these serotypes? AAV6 offers broad tissue tropism for gene therapy...applications (Figure 4). Check out our blog post on AAV serotypes for more about the tissue tropism of these and...

... Viruses can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms...viruses can be produced in multiple different cell types, including plants, mammals, and insects. In this...

...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...collect will depend on the serotype, as some rAAV serotypes are abundantly released into the culture media...

...more suited for prediction of very diverse edit types and cell contexts. Figure 1: High-throughput... diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016/j.cell...

...light does not overlap in wavelength. In many microscopes the filters are not narrow enough to distinguish...Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent ...

...Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

...contained a viral envelope to dictate the tropism, or types of cells the vector could infect. Finally, the transfer...envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary cells or...

...on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their phages ...Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria. It’s a...

...location, location, location There are many different types of samples at Addgene - DNA sent from depositors...barcodes additionally allow us to store multiple types of tube for each plasmid we have at Addgene. Each...