...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...labeling experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures...

...simply embellish and adorn your lab space or your work from home space in a fun, festive holiday or winter...winter or holiday theme decor in your work from home space… but with a science twist. Maybe you have a collection...using life science catalog pages. Make your WFH space feel a little more festive this holiday season! ...Instagram as we share pictures of our decked out spaces.       ...

...versions obsolete, the techniques we use in lab are replaced by improved versions that save both time and money...observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...unknown sequence. In the era before sequencing was commonplace, researchers used restriction mapping to investigate...this technology, radiolabelled nucleotides were replaced with fluorescently labeled ones allowing for fluorometric-based...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful applications...to recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly...

...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching from ...that bring more to the table in terms of data traceability and reproducibility of research. Electronic ... enables researchers to store everything in one place. From pictures to excel sheets, researchers today...enable researchers to keep all their data in one place and avoid scattering intellectual property across...run, ELNs enable labs to keep their data in one place and therefore make it easier for them to track and...

...non-scientists alike will march to advocate for science’s place in education, government, and civilization in general...attribute that enables science: “humility in the face of evidence” (4). Frustration among scientists has...aspects of research at National Aeronautics and Space Agency, the Environmental Protection Agency, and...inclusion of science) they come from many different places supporting many different positions. It would be...and 514 satellite marches are scheduled to take place around the world. There is no requirement that marchers...plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s first budget Environmental ...

...(Brinkman et al., NAR). This technique uses the trace file generated in Sanger sequencing and a simple...Fig. 1: Overview of TIDE. The input is sequencing trace files of WT and edited cell lines. Then decomposition...and your Cas9-targeted cells. Simply upload both trace files to the TIDE tool online along with your sgRNA...outlined above), except that it uses a 3rd sequencing trace file from the donor DNA. This method can precisely...edited sequence would look like (the 3rd sequencing trace). While it requires a little extra wet lab work,...quantitative assessment of genome editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume...

...world continues to face global challenges, we hope to empower those that need to face these challenges ...global challenges. However, these same researchers face significant barriers to scientific discovery and...near future, we hope to eliminate much of white space on this map and increase plasmid sharing across ...Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn...

...interesting, much of what a phage faces as it approaches a host cell surface are complex polysaccharide structures...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...colonies overnight), flip the membrane over and place it colony-side up onto a fresh LB plate containing... is done, peel the membranes off the plates and place each onto a lysis solution-soaked filter paper (... blocking solution (around 108 CFU/mL is a good place to start) and add your membranes to the solution...

...your Addgene account.  Orders do not need to be placed immediately upon request approval. Once approved...request will remain in your account until you have placed your order. You will only be charged once you have...  What happens after I order?  Once an order is placed, the Addgene viral vectors team gets to work! They... before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for the service... you’ll be able to work with the distributor to place a request and order if approved.  I want to stay...

...researchers from different locations. Having virtual face-to-face meetings as opposed to phone calls most closely...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...Scientific conferences and workshops are another great place to meet up with collaborators. Being able to present...