We narrowed to 762 results for: SOM

...best practices in the field, and even getting into some active science communication research.   This summer...as laypeople when it comes to molecular biology.  Some lay people have a high amount of knowledge or interest... the communication method, that determines if something is scientific or science communication. A Tweet...

...common E. coli strains like DH5-alpha are recA-, some strains have been engineered to further reduce recombination...is important to not overgrow the plasmid at 37°C. Some researchers find that using a 30°C incubation temperature...for single colonies and testing multiple preps. In some cases, we suggest gel extraction to isolate the ...

...Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they guarantee certain...receive that legal agreement. Below, you will find some easy talking points for broaching this subject with...research institutions TTO: Technology Transfer Office. Some synonymous terms include: legal office, office of...

...genome inside a protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...the cell like baby spiders from an egg sac. (Okay, some enveloped viruses bud out, instead of lysing, but...

...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...instructor at the college level, perhaps consider using some elements of BioBits to transition your undergraduate...educational uses. We believe that biology education is something that should be available to everyone, and hope...

... that’s compatible with your experimental setup. Some experiments require higher gene expression and therefore...How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce in high...ways you can optimize AAV production. We’ve listed some simple steps you can take below but you should read...

... or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings...are a number of other analysis scripts available, some associated with specific libraries, but any analysis...amplification. I hope this post has helped clarify some of the important considerations when using, amplifying...

...Read the first post here. Once you’ve identified some fly stocks and other reagents of interest, the next...next question to ask is what to do with them. In some cases, there might be a mutation that disrupts the...running with fly lines that are already available, sometimes you’ll have to make your own. In my next post,...

...part time or full time) and what life is like for someone who currently in the position. Questions you’ll...well as work history can really show that you have some of the abilities listed above. Showing that you ...out our other career posts for more specifics on some careers and to find advice on crafting the perfect...

...through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...(recombinant) monoclonal. Another reason is that some antibodies may just not perform as well in one application...

...scientists. In my time at the meeting, I've made some observations regarding what makes this group of ...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. .... 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, Addgene ...

...skim the manual. Overall, it’s a great tool for someone starting cell culture work. 3. NEB Tools by New...properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids plus ...chain, except if their names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook...

...with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...Phytochromes are light-responsive systems found in some bacteria, but not E. coli. They fall under the heading...

...that the choice of fluorescent proteins requires some thought. The first step in choosing fluorescent...together but CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins. ...proteins or marking compartments. I hope this sheds some light on multicolor imaging with fluorescent proteins...

... down the hall, it is always a good idea to take some time to confirm that you are working with the correct...strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI) are capable of...lanes look like your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA...

...used working definition of systemic capsids. After some consideration, we offer this definition: A systemic...systemic capsids are engineered, as well as highlight some considerations for their use. Then, we’ll provide...differences occur not just between mice and NHPs; some capsids show both different efficacy and tropism...2023 AAV-PHP.S* PNS, including ENS (broad): somatic and visceral sensory ganglia, type II spiral ganglion...E. E., Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli...

...inventory of ready-to-use viral vectors. Here are some of the AAV and lentiviral preps we have released...proteins at the beginning of April. Now, we've turned some of the most requested plasmids into lentiviral preps...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...

...closer look at the construction and validation of some of these tools created by the Trimmer Lab that will...of the correct clones, it was determined that for some constructs more than 90% of the colony PCR validated...aberrant IgG light chain transcript was expressed by some of the hybridomas and, as a result, subsequently...

...lessons to teach, and how — some choose to run full semester-long classes, some pick and choose a few lessons... they remember the moments when they discover something new for themselves and when they effectively contribute...

...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...factor (F-factor). The F-factor is found on an episome, a piece of DNA that can replicate on its own or... or be integrated within a bacterial chromosome and allows the donor bacterium to make a small “bridge...

... in some systems, rare codons and four-amino acid frameshift codons are also used. Note that some systems... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a ...

...hard-to-reach organs, such as the brain. Secondly, some viruses have a low immunogenicity which makes them...Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of Insertional Mutagenesis) High ...Expression High Immunogenecity AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity...

...things to come, we wanted to take a look back at some of the contributions from our depositors. Below ...puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459) V2.0 was...puromycin cassette that makes it less efficient in some cell lines so it may be best to go with pSpCas9(...

... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...each of the three social media platforms, there’s some statistics on what percent of scientists and engineers...

...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...more reproducible and your results more valuable. Some journals, including F1000Research, also ask for ...published. In summary, it is a good idea to take some time and look into which journal would be best suited...

...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...

...reporters stain cultured cells and tissue sections, some reporters are better suited for particular applications... 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies  stain poorly when tagged with a ...secondary Enzymatic reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain...

..., we wanted to walk through some of our favorite milestones and share some thoughts from the community...began distributing ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, ...

...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...applications and have similar advantages, though with some caveats.  First off, F(ab’)2 fragments are bivalent...less stable and have lower binding affinity than some of the larger fragments.  Think of antibody fragments...

...to troubleshoot the cloning experiment. Here are some tips that will help you with your cloning project... Before diving into the experimental work, spend some time outlining the construction of the plasmid and...plasmid troubleshooting guide, you hopefully have some new strategies to tackle that tricky cloning project...