...-defined contours. (Many of them are software engineers, surprisingly, who have deep technical knowledge...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog...

...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

...sciencewithtom.com.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...discoveries from her lab. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     References...

...Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...

...details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,...

...kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging  to Design: FRET reporter activity depends on...

...missing out on an opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an ...

...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...labs around the world. Thank you! It's been a challenging and rewarding journey since starting Addgene....

...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...

...available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments.  Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ...

... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

... However these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by...

...which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...blog post. References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou...

.... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...-ME) is a methylation editing tool from Albert Cheng’s lab that enhances RNA-guided methylcytosine editing...

...development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types...

...trained to be good at learning new things—this is a strength you can draw on and demonstrate in your application...hiring manager. Learn to confidently describe your strengths in your resume and don't be shy about asking for...

... energy and to choose institutions with allies engaged in training excellence to begin effecting change...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to...

...antibody tentacles may have an impact on this challenge either for magnetic capture or for capture using... a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl ...

... focused on scientific outreach and community engagement. Information about contacting Addgene can be ...office in London, Emma Markham. Emma was born in England but has since studied and worked around the world...

... your experiment is definitely not a fluke.” 3. Engage in various projects and help colleagues with their...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...

...selection screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...

...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...and CRISPR History and Development for Genome Engineering  ...