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We narrowed to 796 results for: arc

Showing: 551 - 600 of 796 results

  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV genome design...Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of...

  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... antibody in the lab, you’ll therefore want to research your conjugation reaction to understand if any... an experiment with many proteins of interest! Research the brightness of your fluorophores before buying...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...improve the utility of CRISPR/Cas9 in new avenues of research. The use of multiple gRNAs targeting a single ...editing. Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression...

  5. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...attendance list. Plan the sessions you will attend. Research the speakers in advance and make a note of those...and promotions.  Follow academic scientists on ResearchGate and/or via Pubmed alerts. Congratulate your ...

  6. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style.... Alumni who enter their information in these searchable databases are usually offering their contact ...

  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... (TALEN) kit consisting of 834 plasmids. The researchers showed they could use their TALEN kit to quickly...spoke to Musunuru about how the new kit works, the research the kit now makes possible, and how it compares...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...make room for a gene of interest, AAVs used in research have their genomes “gutted”, meaning that all ...trunk” space for genetic cargo. Typically, when researchers speak about AAVs, they are actually referring...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...to sense their formation, and how clinicians, researchers, and even farmers are harnessing these molecules...the introduction of dsRNA. Synthetic dsRNA Researchers have been harnessing the power of dsRNA for a...

  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...former Caltech researcher, now Director at the Max Planck Institute for Brain Research in Frankfurt am...

  11. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...coli biotin ligase BirA* from BioID and allows a researcher to identify proteins that bind an RNA motif of...biotinylated background proteins reported by other research groups that performed BirA*-based affinity purification...

  12. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...without disrupting protein function. A literature search can help determine which terminus is favorable ...the ENL YEATS domain in acute leukemia. Nature. March 9 2017. 543(7644): 270-274. PubMed PMID: 28241139...

  13. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...offerings. The viral vector team is publishing research, hosting conferences, and are expanding our catalog...on the Addgene website Find plasmids for your research  Learn about our viral service Check out job openings...

  14. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...greatest’ variants with the best properties for your research. But be aware that the fluorescent proteins reported...under the conditions that are relevant for your research. This will reveal the performance of fluorescent...

  15. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...and bind proteins. They’re used in a number of research and clinical applications. Most of the time, when...You never know what problems will arise in your research or what data you may need to collect. Understanding...

  16. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... carefully track samples through our lab using barcoded tubes and plates. The critical pieces of information... to Verify Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...the accumulation of errors in DNA sequences. In research, these errors can affect experimental processes...importance of QC Plasmids are a fundamental part of research. They are easy to produce, easy to edit, and easy...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...! First, a quick refresher: prime editing is a “search and replace” genome editing technique that relies...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...

  20. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...mutant allele. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...traditional gene therapy pipelines (viral), and researchers are currently working on giving this treatment...

  21. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...

  22. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...gleaned from the literature and engagement with researchers who study the protein or structure of interest...introducing tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging...

  23. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...This post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina ...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...

  24. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing reads to...up the run making sure to note the appropriate barcode for each sample. About 24 hours later we unload...

  25. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...

  26. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...refractory to gene delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM...plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA delivery...

  27. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...selection and expression in your target cell line, researchers often find themselves faced with a roadblock....cell line that is used for titering. Typically researchers will choose a relatively permissive cell line...

  30. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity...fluorescence. Image from Eastman et al., 2020. The researchers tested several scenarios from embryo development...

  31. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...

  32. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...Eric Green, Director of the National Human Genome Research Institute (NHGRI) of the NIH, started off with...acid science still has many surprises for the researchers in this field. Resources at Addgene Browse ...

  33. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ...

  34. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007...

  35. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  36. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...

  37. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  38. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

  39. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...

  40. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

  42. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...

  43. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers... Addgene.org Find mammalian plasmids for your research Find viral vectors for infecting mammalian cells...

  44. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... of a gene exists, there must be assays that researchers can use to quantify said copies. They do so using...commonly available and may not be an option to all researchers. Accuracy needed Do you need to call exact...

  45. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...set out to fill this gap by highlighting that researchers learn many transferable skills while working ...

  46. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

  47. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...

  48. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...McDade for creating the Expression Systems table. Marcy Patrick contributed to the writing of this post....

  49. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...for gene knockout, activation, repression, and barcoding.  3) Are you trying to target every gene in the...thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing CRISPR libraries...

  50. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...
Showing: 551 - 600 of 796 results