We narrowed to 329 results for: AGA

1. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   Published

   July 14, 2021, 1:15 p.m.

   ...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold...

3. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How...

4. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ...enable you to quickly BLAST your sequencing result against the expected sequence for quick verification, which...

5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a ...

6. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...the parent cell population using the library, propagated it, separated it into roughly identical replicate...

7. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...

8. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...

9. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...

10. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

11. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

12. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...

13. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

14. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

15. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,...

16. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

17. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...

19. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...

20. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...