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We narrowed to 504 results for: CIT

Showing: 221 - 240 of 504 results

  1. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...though that’s what I thought,” she said. “I’m so excited to see where my life will take me. I’m also just...t had these conversations. Now, I am much more excited about where my career path will take me and much...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

  4. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  5. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...

  6. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

  7. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...

  8. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...

  11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

  12. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express... Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new eBook...

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...efficiencies of 90-100% (Fig. 2B), and >99% on-target specificity. Additionally, by expressing multiple crRNAs ...the CRISPR toolbox RNA-guided transposases are exciting new additions to the growing CRISPR toolbox for...

  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...where limited expression of Cas9 is required and specificity is a concern, such as knockout generation or ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...

  17. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

  18. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...of concentrations but suffers from decreased specificity, especially as it approaches the minimum and ...are more disheartening than seeing a potentially exciting result quashed by a bad standard curve. Take the...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular... mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...

  20. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...
Showing: 221 - 240 of 504 results