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We narrowed to 514 results for: CIT

Showing: 221 - 240 of 514 results
  1. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...for our newsletter and a free plasmid) were very excited about our new viral services. If you’re not familiar...educational content on the blog. Overall, there are many exciting things going on in the world of neuroscience and...
  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...starting our viral vector service in 2016...we’re excited to help scientists share their reagents with the... for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years ...
  3. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...
  4. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...internships share a common set of criteria such as citizenship status, academic year, and grades that serve ...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ...
  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016...
  6. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...big plans for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...
  7. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...
  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...the development and metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy... promote microenvironment’s malignancy but also incite different organs to become malignant. Exosomes ...
  9. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...protein to create three distinct colors. The specificity of these orthologs is key: since each ortholog...
  10. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...
  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...
  12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...though that’s what I thought,” she said. “I’m so excited to see where my life will take me. I’m also just...t had these conversations. Now, I am much more excited about where my career path will take me and much...
  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...
  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...
  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...
  16. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...
  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...
  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...
  19. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story...
Showing: 221 - 240 of 514 results