We narrowed to 514 results for: CIT

...AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and would like to get it working in microglia...development of more and smaller CRISPR tools is an exciting area for future AAV application to epigenetic ...

...possible. This advancement could be especially exciting for scientists working to visualize multiprotein...tools to include nanobodies. At Addgene, we are excited to be part of this initiative and to continue to...

...perform retrograde tracing, but often at the cost of toxicity, low expression, or poor scalability.  Scientists...neurons within their natural environment, all while reciting their favorite slogan, “look how cool the brain...

...Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement authentic scientific...His dissertation and research background is in citizen science and behavioral ecology of cichlid fishes...

...active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016...

...the development and metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy... promote microenvironment’s malignancy but also incite different organs to become malignant. Exosomes ...

...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...protein to create three distinct colors. The specificity of these orthologs is key: since each ortholog...

...big plans for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...

...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...

...though that’s what I thought,” she said. “I’m so excited to see where my life will take me. I’m also just...t had these conversations. Now, I am much more excited about where my career path will take me and much...

...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...

...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...

...in-depth In other tools, guides are ranked by specificity of targeting or by cut-to-insert distance for...determination (CFD score) is used to measure specificity of a guide to the target. The CFD score ranges...

...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story...

...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...

...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...