We narrowed to 402 results for: LARGE

1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...

2. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

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   Sept. 26, 2019, 12:50 p.m.

   ...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or...

3. Tips to Make the Most of a Scientific Conference

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   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...the conference you are attending on the larger side? At large conferences, chances are that several talks...

4. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...indicating that Met protein functions have been largely conserved since the species diverged. Tips on using...

5. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ... commonly observed with direct stimulation are largely absent in the soCoChR-expressing system. Taken ...

6. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...you want to use in a given experiment. For very large panels (upwards of 30-40 colors), spectral flow ...particularly useful tool for those wishing to run larger panels: five laser spectral flow machines have ...fluorophores. This allows for the creation of much larger panels, as fluorophores that cannot be distinguished...

7. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...could be good choices if you have a particularly large number of inserts to assemble. You could also consider...

8. CRISPR in the Clinic

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   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...at two sites within the viral genome to excise a large portion of it. This ongoing trial is sponsored by...

9. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ...

10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ... and time-consuming to generate, especially for large animals such as primates. Viral vectors with cell-type...

11. 3D Printing Meets CRISPR Cas9

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    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...Steitz was presented with a physical model of his large ribosomal subunit, he kept sliding his palm across...the models we create are around 30 million times larger than the real thing. Nevertheless, the models are...

12. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...the possibilities can seem overwhelming given the large assortment of fluorescent proteins available. Fortunately...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    [email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...

15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...

16. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm).  Types of transfers...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes...

17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...proteins are split between strand 10 and 11 to yield large (1–10) and small (11) fragments. In several cases...

18. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...amplicons.  Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ...

19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...relies on antibody chains, which are relatively large and are not expressed consistently throughout cells...

20. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...you are interested in viewing which has drawn a large crowd, making it difficult to see the actual poster...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...