We narrowed to 798 results for: REP

...facilitate repair. Since the development of CRISPR-mediated base editing (BE), these types of repairs can now...the resultant U-G pair is either repaired by cellular mismatch repair machinery, making an original C-...possible through exploiting the homology-directed repair (HDR) pathway which occurs in cells at low frequencies...or reverted to the original C-G by base excision repair mediated by uracil glycosylase (Figure 1). The ...pair (Figure 1). As the cellular inosine excision repair is not as active as uracil excision, ABE does not...al. constructed a number of plant base editors replacing the SpCas9 with the mutant versions of SpCas9 ...

...and placed in the legend. Widely reported difficulties in reproducing experimental data are likely, at...readers may locate data supporting the research reported in PLOS publications. The policy was not an overnight...author. Instead, selected images are said to be representative or the statistical variation is calculated ...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved by...Scientific Data, which aims to act as a peer-reviewed repository for “scientifically valuable” datasets. It likely...likely won’t be long before other data repositories emerge that allow efficient searching and extraction... in our interest to ensure that our data are reproducible and useful to others and efforts to enhance ...

...painstakingly assessed to something a bit more representative of the communities’ opinion. The trouble is... The foremost handles the design of sgRNA and represents the lion’s share of the currently available tools...a good example is CRISPR-GA. These tools assess repair events by type (NHEJ/HDR) and track indels at a...no longer available), is a simple catalogue of reported sgRNA designs. The second database, CrisprGE (...speed up the sgRNA design process by removing repetitive tasks and/or by providing features to help with...it takes authors to add a genome to the tool’s repertoire. Organism agnostic tools are the better solution...to learn the language and what the parameters represent. The best learn-by-doing tool is E-CRISP. The ...

...resistance cassette, and the R6K origin of replication to allow replication only in hosts carrying the bacteriophage...mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile technique...recipient of interest. Because pDS132 cannot be replicated in the recipient, the only antibiotic resistant...modifications to pDS132 (Philippe et al., 2004): Replaced the sacB cassette with one of three toxins from...the Salis group) (Espah Borujeni et al., 2014). Replaced the pDS132 MCS with a versatile, synthetic MCS...recombineering enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., 2016), since...fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found to be a...E., Sivanesan, D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein...Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions...evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu, D. et al...

... are tagged with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe...DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence upon cleavage. Upon ... results in the trans cleavage of the ssDNA-FQ reporter resulting in fluorescence. Cas12a binds all the...results in the continuous cleavage of the ssDNA-FQ reporter. The resulting fluorescence can be observed under...fluorescence visualizer with 9V battery Approach :This preprint uses a previously developed method to detect SARS-CoV...biotinylated primers. These are then immobilized with streptavidin coated beads. Fluorescently labelled dCas9 complexes... concept to lateral flow strips that contain streptavidin which can bind to the biotinylated targets. ...

...sequences required for viral replication and transcription as well as reporter genes. To learn how you can...include activation and repression of your target gene, activation and repression of different genes in ...Lab at MIT developed plasmid based systems that report the order in which specific chemical inputs are...recombinase-based state machines could potentially be used to report upon and/or engineer “temporally ordered biochemical...derived from Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to...

...as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template...new era in C. elegans research. Homology directed repair (HDR), which enables insertion of custom genomic... a fluorescent protein fusion, transcriptional reporter, and loss-of-function allele in just one injection... CRISPR/Cas9 applications, SEC selection could replace other, more restrictive C. elegans selectable marker.... This novel, rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution...Editing Efficiency CRISPR 101: Homology Directed Repair  ...

... available at Addgene, either as in-stock viral preps or through our Packaged on Request service. Alternatively...for each serotype below) and make your own viral prep. However, tissue tropism varies both between species... targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial... muscle inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction... the CNS (Han et al., 2023), and has also been reported to have tropism for liver and kidney cells (Issa...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...cells utilizing various AAV serotypes. Scientific Reports, 15(1), 20927. https://doi.org/10.1038/s41598-025...

...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles....-surface protein labeling and identification. Reproduced from Wu et al. (2025) under CC BY license. ...Grelet Lab developed MitoTRACER, a retroviral-based reporter system that permanently labels the cells receiving... slice expressing PinkyCaMP; scale bar 50 µm. Reproduced from Fink & Imai et al. (2024) under CC BY-NC-ND... or SomaFRCaMPi in neurons; scale bars 50 µm. Reproduced from Zhou, Zhu, Eom, Fang, et al. (2025) under...condensates, disrupting their dynamics and composition. Reproduced from Zhang et al. (2025) under CC BY license....using either "high-tech" or "low-tech" equipment. Reproduced from Collins & Lau et al. (2024) under CC BY-...

...will amplify even if its target states are only represented in a very small number of registers. Instead,...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...The input plasmid (high copy, ColE1 origin of replication), contains large serine recombinase genes downstream...we used to drive recombinase expression may be replaced by sensors that correspond to the desired inputs...

...published a report on the social and ethical issues of genome editing and human reproduction, the relevant...Nuffield Council’s report is that when it became clear that this assistive reproductive technique was feasible..., the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/...question of “how do we move forward” is the June 2012 report on the ethics of “Novel techniques for the prevention...established ethical issues raised around mitochondrial replacement therapies. In other words, rather than having...

...luciferases, which have become common genetic reporters and alternatives to fluorescent proteins. ...Created with BioRender.com. Bioluminescent reporters require a chemical substrate (and sometimes cofactors...signal sensitivity. Another advantage: luciferase reporters aren’t susceptible to photobleaching — they’ll...firefly luciferase was the first bioluminescent reporter to be cloned and remains popular, other luciferases...Engineered   677 61 AkaLumine ATP No Luciferase reporter assays for monitoring gene regulation Since firefly...approach has even been used in biosensors where BRET reports on the change in conformation of a single protein...expression vectors, and literally thousands of reporter constructs with a gene of interest already inserted...

... These breaks can then be repaired by homologous recombination with a repair template.  Each zinc finger...Frederick Griffith used mouse models to prove that Streptococcus pneumoniae bacteria could be transformed from... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...genetic engineering as CRISPR-Cas editing soon replaced the protein-based DNA-binding domain with a single-guide...genome. These technologies rely on the cellular DNA repair machinery, which can lead to high frequency of ... Newby GA, Raguram A, Liu DR (2019) Search-and-replace genome editing without double-strand breaks or ...10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular Structure of Nucleic Acids. Annals of...

...the ligation junction were represented. Sixteen separate pools were prepared, each with a different complement...fidelity. In this method, substrate pools were prepared consisting of one target (template) strand and...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...routine cloning or DNA library preparation. However, when a method relies on accurate ligation of nicks... Biolabs, specializing in the enzymology of DNA repair enzymes.     References 1. Shuman, Stewart, and...et al. "Ligase-based detection of mononucleotide repeat sequences." Nucleic acids research 27.24 (1999)..., and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked...

...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition... built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial...distribution of gut bacteria without fixation or preparation, and deliver CRISPR components for gene disruption...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...

...able to replicate, and for a virus to cross a synapse from one cell to another, it must replicate. Modified...infects one cell (the “starter cell”). It then replicates within that cell, and nascent RABV cross synapses...infection occurs, subsequent cycles of viral replication and retrograde transmission occur, spreading ...limitations. First, these high levels of viral replication are toxic. Second, infecting subsequently higher...G-deleted RABV will infect the starter cell. RABV replication will then occur and the G protein (supplied in...order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated by other viral...

... have all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or... sufficient quantity of DNA is produced from a miniprep and “low copy” if special growth conditions are...cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria...For plasmids that contain the R6Kγ origin of replication, choose the Pir1 strain. If your plasmid cannot...in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send the... you a deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your...

...chemical replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no...pathways are involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance...inheritance that might be less error prone than DNA replication. He too mentioned how “surprising” small RNAs...water. Dr. Terns gave a great introduction to the repeated and distinct aspects of the CRISPR regions of ...gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria thus... labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine...

...first scientific poster, but unsure how best to prepare. Maybe you’re a presentation veteran, but have ...experience. No matter the reason for attending, your preparation and day-of game plan will ensure you have the...experience possible. Tips for poster session presenters Prepare for success You’ve put a great deal of thought ...it’s time to expertly show off your science.  Prepare the night before: To avoid rushing out the door...poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our posters... part of a poster session, but with a little preparation and a few deep breaths the day-of, you’ll handle...Strategically traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve...