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We narrowed to 580 results for: RON

Showing: 221 - 240 of 580 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316... Michelle Cronin...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... affinity terminator may be desired for multi-cistronic constructs where high termination efficiency is...Bacterial Transcription Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297...Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional...

  3. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ... 0 and 100 for each guide, with 100 being the strongest interaction between the guide and the target and...interactions (e.g., scores <20) are reduced to 0 and strong interactions (e.g., scores >80) are transformed...weak gRNAs (CFD < 20) bind almost not at all, and strong gRNAs (CFD > 80) bind almost as much as perfect...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...possible because baculoviruses are slightly less prone to recombination than other viruses, like lentivirus.... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https:...

  6. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...Compensation beads are a convenient tool to obtain a strong positive signal for a given fluorophore, as they...won’t bind anything (negative population). It is strongly recommended that when you first run a new panel...compensation beads are convenient in that they provide a strong positive signal, even for rare cellular markers...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...genomic site can be edited based on its chromatin environment. After selecting the best pegRNA design with ...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid...

  8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there...technology: because they necessarily alter the shared environment, they must never be used without popular support2...organisms and associated ecosystems.  Because the environment is shared by everyone, he is deeply concerned...

  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...adenovirus-based gene therapy clinical trial gone horribly wrong. It brought the whole field to a grinding stop and...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...Applications of CRISPR-Based Genome Editing in the Retina. Front Cell Dev Biol. 2018 May 14;6:53. Review. PubMed ...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...cost-effective, timesaving for transformations. Cons: Prone to satellite colony formation on agar plates, less...less stable at high temperatures and in acidic environments.   Carbenicillin   Carbenicillin, colloquially...antibiotic than amp: it is more stable in high pH environments and is less susceptible to degradation in agar...

  12. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...resulting HeLa cells show robust labeling, with strong expression and detection of each fluorophore at...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antibiotic resistant cells could repopulate the environment after treatment. The phagemid systems used in...pathogenic or drug-resistant bacteria. It’s a bit ironic that CRISPR, the bacterial immune system, may someday...4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron. “Temperate and lytic bacteriophages programmed...

  14. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work/life balance.... spouse won’t be at every school performance, chaperone every field trip or cheer at every soccer game...birth and breastfeed. Keeping this always at the forefront will go a long way toward alleviating the guilt...

  15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced...Ly6a seemed the most likely candidate due to its strong linkage to the PHP.B phenotype and its expression...references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive gene transfer to the ...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...analysis window shows a green checked circle in front of the sample name. If there was a minor error during... error, you will see a red exclamation point in front of that sample. You can hover over the yellow or...line represents the cut site. Cutting and error-prone repair typically result in mixed sequencing bases...

  18. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...research for over a century but was brought to the forefront by Thomas Hunt Morgan, who is known as the “father...work with as they can survive in a variety of environmental conditions, and double every 2 hours. Yeast ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...Jonathan. "RNA fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy... and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...
Showing: 221 - 240 of 580 results