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We narrowed to 749 results for: SOM

Showing: 221 - 240 of 749 results

  1. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...expertise with lots of free beer.” He also mentions that some of the team will be moving to flats with gardens... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family... family. And as the school year winds down, take some time to have fun outside the lab! How does your ...

  2. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...parameters of CRISPR experimental design. Let’s dive into some structural biology! Major features First, we’ll ...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...7S4X), shown opaque (left) and transparent (right). Some nucleotides of the non-target DNA strand are not.../doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima...

  3. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...every two weeks or every month is long enough that some experiments can get done and data analyzed between...scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...the output that will come from the collaboration. Some things to figure out are timelines for experiments...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog...

  4. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...drug downstream. In some cases, there may already exist a drug which targets some of the candidate genes...onset. These hits can reveal individual genes or sometimes even genetic pathways that are worth investigating...drug development. Due to the difficulty of drugging some targets – the dreaded ‘undruggable’ proteins – having...

  5. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    Published
    Oct. 3, 2017, 12:11 p.m.
    ... in scicomm has grown and now it feels like an awesome bionerd hobby but also a viable away-from-the-bench...was leaving a writing position and looking for someone who might take over. I like writing and science...communicator than a science writer these days. I do some public speaking and a bit of video work for webinars...after joining IDT when his boss was looking for someone to take over hosting them. Since then, webinars...

  6. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...thought we’d take some time to introduce you to the idea of the Freshers Fair and some of the great biological...scientific networks in their many years of study to come. Some Freshers Fairs can include fairground rides and ...know of a society or event which would benefit from some Addgene support? Please get in touch, as we aim ...

  7. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...reporting on amazing women in science, or asking someone how and why they left academia. Humans are designed...research. Long story short, telling a good story is something that can be learned, and will serve you well in...yourself jumping backwards in time to explain something, rethink your structure. Develop your protagonist...but there are plenty of other options. Choose something with emotional significance that is likely to ...

  8. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...like we’re being buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March... 2020 after rumors spread in our building that someone from a different company had tested positive for...as you are able... Thanks to everyone for being awesome during a tough time. We're all going to get through...access to quality materials and data. Addgene has some exciting new projects planned, and we look forward...

  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...commonly available tags and their antibodies along with some pros and cons. FLAG FLAG is a synthetically derived...immunoprecipitation, western blot, and immunofluorescence with some success for protein purification as well. This tag...a protein may disrupt the biological function of some proteins. Protein folding issues, steric hindrances...

  10. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... most chromosomes are represented by two to three copies, but several have four. No Y chromosome is present...gland is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t ...advantage to use the cell line and we will highlight some of those applications here.   Virus production HEKs...

  11. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...with the condition to reveal some “outstanding differences.” They now have some theories on the underlying...on rare diseases, offering them the chance to win some of the latest tools and technologies in life sciences...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012...

  12. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...multibillion dollar tech companies using only laptops and some server space. But what if you want to be an entrepreneur...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...experiences founding and running miLab to describe some key lessons I learned while becoming a health science...

  13. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...genetic tools are available. While he recognizes that some beginners may have a tendency to shy away from ratiometric...he recommends they give it a shot. “Beginners sometimes think it’s complicated, but actually it is very...of success – whether we have really developed something useful [enough] that other people start using ...Check out our Hot Plasmid page, where we highlight some of the popular & newly available plasmids at Addgene...

  14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ... mentor, developed some strategies for finding mentors and, perhaps, asked someone to support your career...identify areas that need work or growth. Here are some ideas for making your meetings productive: Discuss... posse holds me accountable for attending and sometimes it is only the knowledge that they will be watching...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...students! After a course coordination meeting with some other professors, I went home thinking about how...When I got home, I saw my daughters playing with some cards and I had a quick flashback to time spent ... most of the concepts required by the course and some were so enthusiastic that they took the cards and...gamification” will lead to new cures for disease someday! Accessing and modifying BUILD YOUR PLASMID You...

  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... news and there’s bad news. The bad news is that some of tubes’ o-rings are, in fact, permeable to CO2...information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples on dry... dry ice - it may prevent you from seeing some unexpected results. Order Ready to Use Viral Preps from... can affect the pH of the sample, which may have some effect on the contents of the sample. For example...

  17. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...take responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately...Specify desired results. Are you delegating to someone with sufficient skills and experience? Do you think... think you are giving this responsibility to someone who can do the job. It is good to delegate so the...and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea. How...

  18. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for some quick advice and resources for hiring research staff... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...The Howard Hughes Medical Institute (HHMI) offers some great resources for Early Career Scientists, including...

  19. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...that didn’t get answered live - but he kindly took some time to address all your unanswered questions via...be oversized even before considering the need for some manner of polyA. In this scenario, you may be best...adopting ddPCR, which does not a standard curve and somewhat more reliable quantitation. Differences in sample...VAST) experiments using PHP.eB in the brain give some insight into codelivery efficiency at different ...

  20. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...manufacturer, lot number, and expiration date somewhere you can easily check it whenever you use the antibody...freeze-thaw cycles, as they’ll degrade your antibody.  At some point in your long and illustrious lab career, you... with other chemicals and biological structures, some of which you may need in your experiment.  If you... to buffers, can bind to amine groups present in some conjugates, blocking the conjugation reaction. Many...
Showing: 221 - 240 of 749 results