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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 281 results for: hal.1

Showing: 221 - 240 of 281 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...

  2. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas effector (Cascade...s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...

  4. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent proteins were best ...with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene #104106...

  6. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently...doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the need for target-specific...

  7. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein transduction: unrestricted...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate ...

  8. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed. Find the Plasmids @Addgene...However, these approaches are often met with the challenges of regulatory bottlenecks and their scope is ...

  9. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to...was researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... inhibitory synapses in the brain.  Figure 1:Schematic diagram of different FingR constructs and...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...H2B-FT-red is long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts...

  12. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...treatment and improving patients lives. References 1. Ong-Abdullah, Meilina, et al. "Loss of Karma transposon...real-time, posing a huge challenge to studying epigenetic changes over time.  This challenge was difficult to ...

  13. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...supervisor, even if he or she is a difficult person: 1) Understand your supervisor’s expectations Every ... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

  14. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault...solutions to complex challenges. As a cross-disciplinary researcher, the challenge of keeping up on studies... Sharing your research with the world can be challenging. After months, or years, of grueling effort to...biology or international relations.  Many of the challenges in science today are too complex for any single...making publishing their research all the more challenging even when it is of quality). On the site you ...

  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...therapies to clinical trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2 protein...cassette using tamoxifen-inducible Cre not only halted the neurological damage but completely reversed... Rett Syndrome, respectively. One of the main challenges facing Rett researchers is how to deliver the...adeno-associated viral (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal... by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches, such...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...yourself in a creative rut, read on!   Fig 1: Blugene using creative problem-solving in the lab... needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding ... review it. This survey generated 26 ideas, over half of which, when implemented, impacted the entire ...you can, set aside time to prepare for future challenges. This doesn’t have to be time-consuming or even... keeping your science going throughout these challenging times.  Take a Break! If all else fails, one ...

  17. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... education and scientific writing.     References 1. Martz, Colin A., et al. "Systematic identification...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...faster. Years of science, done in weeks Figure 1: Sending out 80 FedEx envelopes in a single day --...hijacking of human cells. Even better, more than half the drugs hitting these proteins are FDA-approved...

  19. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...guide sgRNA-Sirius-8XMS2 which will recruit the HaloTag with red fluorescent ligands to the loci of interest...

  20. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...regarding what makes this group of researchers special: 1. Stem cell scientists care deeply about ethics Ethics...trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to a spinal cord...
Showing: 221 - 240 of 281 results