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TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....long, whistling while we work. This is certainly not the truth (partially because I can’t whistle). No,...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...
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Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....BFP mutant (BFPH66) contains a histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon that...C-to-T base editing event. This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m.... Commons license. Histograms For analysis of a single marker, a histogram showing frequency of events... “true” signal. Figure 8: A series of histograms from Alyamani et al., 2018, showing number of... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m.... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds...and using an epitope-based strategy such as Anti-6xHis. You could also consider strategies using other ....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog PostPublishedNov. 16, 2020, 2:15 p.m....also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013). , with the addition of histone modifiers, ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....We do this by promoting citation, supporting sophisticated search, and curating plasmid collections. Addgene's...whether the plasmid has been used in your system. Sophisticated search You can search for specific plasmids ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....for billions of years (…) Bacteria are really sophisticated”. New to CRISPR/Cas9 Technology? Learn ...Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution A Powerful... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS cells. They then showed that...frankenbody probe could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m.... polymers that can work with all the bells and whistles. The fibers are made from a process called thermal...additional components are added - the ‘bells and whistles’ – including microscale light emitting diodes,... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....technology or as a therapeutic. A (very) brief history GCE came onto the synthetic biology scene in 1989... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....in C++ and Java Command line history: Tracking command line history by recording all commands entered... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....technique for monoclonal antibodies. J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...