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TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ...
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Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....BFP mutant (BFPH66) contains a histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon that...C-to-T base editing event. This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m.... Commons license. Histograms For analysis of a single marker, a histogram showing frequency of events... “true” signal. Figure 8: A series of histograms from Alyamani et al., 2018, showing number of... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m.... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds...and using an epitope-based strategy such as Anti-6xHis. You could also consider strategies using other ....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog PostPublishedNov. 16, 2020, 2:15 p.m....also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013). , with the addition of histone modifiers, ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....We do this by promoting citation, supporting sophisticated search, and curating plasmid collections. Addgene's...whether the plasmid has been used in your system. Sophisticated search You can search for specific plasmids ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....for billions of years (…) Bacteria are really sophisticated”. New to CRISPR/Cas9 Technology? Learn ...Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution A Powerful... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m.... polymers that can work with all the bells and whistles. The fibers are made from a process called thermal...additional components are added - the ‘bells and whistles’ – including microscale light emitting diodes,... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS cells. They then showed that...frankenbody probe could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....technology or as a therapeutic. A (very) brief history GCE came onto the synthetic biology scene in 1989... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....in C++ and Java Command line history: Tracking command line history by recording all commands entered... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....technique for monoclonal antibodies. J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...