Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 367 results for: his

Showing: 221 - 240 of 367 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ....8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464 (... 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) 465 (...2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein...

  2. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

  3. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...long, whistling while we work. This is certainly not the truth (partially because I can’t whistle). No,...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds...and using an epitope-based strategy such as Anti-6xHis. You could also consider strategies using other ....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...

  9. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid...

  10. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013).   , with the addition of histone modifiers, ...

  13. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... polymers that can work with all the bells and whistles. The fibers are made from a process called thermal...additional components are added - the ‘bells and whistles’ – including microscale light emitting diodes,...

  15. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...

  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

  19. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...technique for monoclonal antibodies.  J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org...

  20. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...
Showing: 221 - 240 of 367 results