...real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be used to detect ...commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing the fragments...

...Traditionally, delivery is achieved through the use of invasive methods, limiting researchers to accessible, spatially...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... Gradinaru, V. (2022). Engineered AAVs for non-invasive gene delivery to rodent and non-human primate ...

...How are their organizational skills? Are they involved with colleagues and the department? Are they ...transferable skills by attending career events or being involved in a grad or postdoc association? Will you learn...

...to perform functional screens to identify genes involved in a phenotype of interest. This blog post will... is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent...

... fragment library of phage DNA This first step involves digesting phage genomic DNA into 1-3 kb (approximately...fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening using compressed...

... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...

...working in a rural health clinic in Ghana. My role involved teaching the nursing students and community health... future supervisors were keen for me to become involved with many different aspects of the company and...

...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...

...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...

...That’s a subtle but important distinction. If I invite someone to be on a project team, I try to make ...next team works better. In fact, don’t wait to be invited onto a team. Make one yourself. Instead of thinking...

...Network blog for more ideas of when and how to get involved.  Why do you do science policy? I would argue...Journal of Science Policy and Governance.  Get involved! The best way to learn about science policy and...

...you should investigate your options and weigh the potential benefits and costs before investing in one approach...

...themselves, use the blood stream to commute to and invade multiple organs, and most importantly escape the...tumor cells and immune cells to knockout proteins involved in immune tolerance. Recently, China spearheaded...

... IV-B have led to the hypotheses that they are involved in plasmid competition or can hijack machinery...Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids...

...lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...structurally characterizing large ion channels involved in skeletal muscle contraction.   References 1...

...editor invented. ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE...

...not in the iso-genic knockout control. This can involve cell surface labeling and/or intracellular labeling...overexpress the target of interest. This approach often involves the transient transfection of expression plasmids...

...shouldn’t be a surprise that the CRISPR system was involved. Today, that same laboratory enjoys a successful... a clear winner, is there something that we can invest our time into learning and extract the most value...

...Measuring pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts...test for mouse iPSCs, tetraploid complementation, involves injecting tetraploid blastocysts with iPSCs and...