We narrowed to 466 results for: invs

...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

...silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes or to knock down ...term for a number of different applications that involve different types of small RNA molecules, such as...

...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

... a suicide switch in cyanobacteria, synthetic inverters in E. coli, and biosensors in E. coli: Čelešnik...017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs." PloS...

...real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be used to detect ...commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing the fragments...

...Traditionally, delivery is achieved through the use of invasive methods, limiting researchers to accessible, spatially...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... Gradinaru, V. (2022). Engineered AAVs for non-invasive gene delivery to rodent and non-human primate ...

...fluorescent and optogenetic tools. FRCaMPi uses an inverted topology of FRCaMP (itself based on mApple, which... components to adipose cells to activate genes involved in converting white adipose tissue (WAT) cells...

...How are their organizational skills? Are they involved with colleagues and the department? Are they ...transferable skills by attending career events or being involved in a grad or postdoc association? Will you learn...

... fragment library of phage DNA This first step involves digesting phage genomic DNA into 1-3 kb (approximately...fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening using compressed...

... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...

...working in a rural health clinic in Ghana. My role involved teaching the nursing students and community health... future supervisors were keen for me to become involved with many different aspects of the company and...

...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...

...to perform functional screens to identify genes involved in a phenotype of interest. This blog post will... is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent...

...Network blog for more ideas of when and how to get involved.  Why do you do science policy? I would argue...Journal of Science Policy and Governance.  Get involved! The best way to learn about science policy and...

...That’s a subtle but important distinction. If I invite someone to be on a project team, I try to make ...next team works better. In fact, don’t wait to be invited onto a team. Make one yourself. Instead of thinking...

...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...

...themselves, use the blood stream to commute to and invade multiple organs, and most importantly escape the...tumor cells and immune cells to knockout proteins involved in immune tolerance. Recently, China spearheaded...

... IV-B have led to the hypotheses that they are involved in plasmid competition or can hijack machinery...Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids...

...you should investigate your options and weigh the potential benefits and costs before investing in one approach...

...lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...