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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 221 - 240 of 628 results

  1. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...access to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant...daily operations for all ongoing processes are entirely self-supporting. We hire more staff as we need...vector distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids ...

  2. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...requires travel can show that you are self organised, reliable, and can work independently. Practical outdoor...are highly desired, as roles are becoming more reliant on technology, and good computer skills will give... every role you need to show that you have the relevant skills and experience.You might not normally think...content in your Resume and Cover Letter. Include any relevant job specific ‘buzz words’ to ensure recruitment...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...site-specifically to alter its function, mimic a biologically relevant modification, or add in fluorescent or chemically...which can help determine structure and function relationships or, in more practical applications, allow a ...tRNA, and the tRNA synthetases. And, naturally, rarely are the optimization parameters the same for all... any of a huge variety of ncAAs to study the relationship between structure and function…if you can get...

  4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...

  5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...resulting in the activation of keratinocytes, which release inflammatory mediators that trigger the activation...pharmaceutical interventions that would inhibit IL-36 release,” says Dr. Mark Mellett, a senior researcher at...autoinflammatory loop: keratinocytes both respond to and release IL-36 cytokines. Keratinocytes are the main source...what molecules or proteins are involved in IL-36 release in keratinocytes,” says Mellett. This would help...

  6. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...This biosensor changes fluorescence based on the release of glutamate from neurons and this new version ...iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at once. “This work has...functional contribution to a variety of behaviors related to addiction and pain. Once these are completed...neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine administration and...

  7. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...into larger units or circuits with functional relevance following a set hierarchy. MoClo originally developed...technique. Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods...libraries depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed...identify the best cocktail of genes when the relationship between genotype and phenotype is best described...

  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... However, concerns have been raised over the reliability of using CNO as a DREADD agonist due to its ability...the compound, or compounds, you choose to ensure reliable results (Goutaudier et al., 2019). Using PSAMs...BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...

  9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...embark on or continue their careers in science-related fields. In particular, two recent events have the...note is a ruling in August by the National Labor Relations Board to allow graduate students who work as teaching...university and alterations to the student-university relationship, along with, of course, union dues. Time will...institutional responses to the FLSA ruling. Similarly, from purely a salary perspective, while this is a move in the...

  10. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...of an outbreak of the virus in Brazil that was correlated with greater rates of infection and rare, but...develop our paper-based platform into clinically relevant diagnostics for the Zika virus was daunting. It...Our approach now detects samples at clinically relevant concentrations (1-3 fM; 1x106-fold lower than ...shown that we can detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... (such as a gene) and initiate the process of releasing the newly synthesized RNA from the transcription... is also known as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA...terminates differently. Polymerase III, for example, relies on a specific sequence and RNA secondary structure...downstream GU-rich region (shown in the figure below) releases the mRNA from the polymerase and creates a free...

  12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...of protein concentration. Calibration curves correlating this ratio to the total expression time can therefore...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...nascent tissues and the formation of limbs are correlated with changes in gene expression. In a developing...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ... Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou...efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. These viral ... could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral CRISPR/Cas9 genome...

  14. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... activation, and knockout. Most CRISPR screens rely on lentiviral systems, a fact supported by the wide...still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo...not integrate into the host genome. AAV instead relies mainly on episomal expression. Episomes are circular...It is important to note, however, that while AAV rarely integrates into the genome, CRISPR may increase...

  15. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...good, high quality preps due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability...Certain strains, including HB101 and derivatives, release large amounts of carbohydrates that can inhibit... a plasmid whose copy number is unknown, it is entirely possible that you are working with a low copy ...these factors are generally, but not perfectly, correlated. Typical growth times for high copy plasmids...

  16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...parts have been carefully engineered to support reliable functional composition. In other words, these ...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity...and to the design community: If you can begin to reliably put together 10-gene systems, what do you want...3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via standard transcription and...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...The genome of viral tools are often not virology related at all, and you will instead get expression of ...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  18. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... If you are miserable at home or work, your relationships will suffer in both the short run and the long...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The... make your life easier and to have more time to relax and be with friends and family. #7 Thou shalt create...swim lesson missed can mean a world of mental relaxation. And, I’m not sure I should put this in print...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...integrating neuronal signalling and triggering release of the stress hormone norepinephrine to promote...are chemogenetically activated, norepinephrine release causes a freezing response, a form of immobility...study from 2014 (Bahi et al., 2014) supports the relevance of BDNF in depression. The researchers found that...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...this information to identify a large variety of related bacteria with similar Cas systems. Some of these...knockdown efficiencies. The ability to identify related Cas systems opens up the potential of further development.... But they weren’t done engineering quite yet – surely, they thought, the integration rate could be improved...with mRNA and a synthetic guide. Like PRIME, PASTE relies on single-stranded nicks instead of the double-...
Showing: 221 - 240 of 628 results