...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only ...It’s part of Google Drive so it has a similar interface as Google Docs or other products in the suite....collection and drag and drop an icon onto the workspace. The color and size of icons are adjustable. New...

...These scaffolds are stretches of non-coding RNA interspaced with linkers that can fold into a secondary structure...this new scaffold RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit...five genes that encode biosynthetic enzymes for violacein, a distinctive pigmented protein. By altering ...generate differently pigmented derivatives of violacein. Using dCas9 as an on/off switch The multiple ...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

...Archives allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article ...

... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed...cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the homogenate and...shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate...

...to almost every person regardless of profession, race, or gender. Almost no other disease receives as ...divide only at the right times and in the right places. Checks and balances: protecting against cancerous...Luckily, there are several safety mechanisms in place to protect us from rogue cells when something does...variety of cancers, Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult...Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer Lineage Tracing ...

...volunteering, it gave me a place to go once a week to get recharged. The fun change of pace helped break up the... great way to share some of the research taking place at our university with the community. I even spray...interact with their followers. It’s also been a great place to reach out to local establishments such as museums...provide plenty of negativity, so I volunteered at a place that made me happy. I started volunteering in the...

...the secretory pathway to their subcellular or extracellular destination. At the end of their life cycle,...to be a part of an organelle or involved in intracellular transport pathways, it is possible to map the... in order for the antibodies to reach their intracellular targets. Therefore, it can be beneficial to ...Transfer, FRET. Additional opportunities: Tracking intracellular pathogens In addition to tracking the location...possible to follow the life cycle of viruses and intracellular bacteria – have a look at our Microbiology resources...

... enzyme 2 (ACE2) cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target...SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used the library to identify RBD...RBD mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV...

...your science, one of the big questions you'll be faced with is "how?" How are you going convey your information...learned (somewhat successfully) how to text or use Facebook. However you choose to communicate your science...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

...REPAIR (RNA editing for programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated ...they confirmed A->I editing and found that 50 nt spacers increase editing efficiency but also increase off-target...