We narrowed to 762 results for: SOM

...to troubleshoot the cloning experiment. Here are some tips that will help you with your cloning project... Before diving into the experimental work, spend some time outlining the construction of the plasmid and...plasmid troubleshooting guide, you hopefully have some new strategies to tackle that tricky cloning project...

...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...genetic designs of that scale could be created. Someone could take on an engineering project including ...

...to quickly identify underlying genetic causes of some human conditions, opening up new avenues for therapeutics...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as...

...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: The ...hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now ...

...methods vary in their transcriptional activators: some methods rely on fusion proteins while others re-... exhibits modest levels of gene activation, with some genes experiencing around 2-fold activation levels...method has shown higher activation levels than SAM in some loci across Drosophila cell lines (Sajwan et al....

...patterns throughout the project where it solidified some concepts that are heard often during undergrad. ...huge interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not... does. Her parents told her that if she found something she loves to do, she would never work a day in...

...forward to where it is today. Below we will review some of the CRISPR-based editing therapies that are currently...CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-phase clinical...CRISPR-HIV therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut...

...the plasmid to stop functioning properly or be something completely different than what was originally ... other critical functional components. Fun fact! Some older plasmids in our collection do not have a full...based on SnapGene’s “Common Feature” library — with some additional features added by the QC team — and allows...

... initiating the desired cleavage event, although some engineered terminators come close (>95%). For most...presence of the poly(A) tail targets the RNA to the degradosome, which contains enzymes that cut RNA not protected...types of RNA: Polymerase I is responsible for ribosomal RNA, Polymerase II is responsible for mRNA and...

...and sequencing is being organized and funded by someone else (KittyBiome has been incorporated as a non-profit...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...And if not, I suspect you can find a makerspace somewhere near you. The place to start looking for your ...

...may be transported through the vesicles to the lysosome where they are finally degraded (2, 3). Once you've...software tools can be used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc...limitations and well-executed experiments require some consideration of FP properties. It is important ...

...fitness cost varied greatly between chromoproteins. In some cases, cells containing high-copy plasmid-borne ...though expressing the chromoproteins from the chromosome could get around this problem. There is no single...chromoproteins are and how they can be used, why not add some color into your research? If you have a favorite...

...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...Although there are a few aspects of spectral flow that some users may find more difficult compared to conventional...

...Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository...function of the distance between measured pixel and the soma. The initiation site identified in panel (C) is ...-independent versions that are trafficked to the soma via the Kv2.1 targeting motif as well as a pan-membrane...

...editing performance, we suggest that users select some of the best designs (e.g., top 5) and test them ...strengths of PRIDICT/PRIDICT2.0 while accounting for some of the inherent inaccuracies of computational prediction...Koeppel et al., 2023), their predictive power is sometimes limited across different targets due to variations...

...achieve. Most pooled library protocols aim for somewhere between 30x and 200x coverage, meaning that you...Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 144-188 bp...closer to your own pooled library. Be aware that some older libraries may not have amplification protocols...

...yourself: I always find it odd when I walk up to someone’s poster, and then he/she dives right into the background... background without an introduction. If someone approaches your poster, introduce yourself and ask about...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...

...visualizing cellular components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses, and cytoskeleton...Every few months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository...

... cellular proliferation. We have appreciated for some time the causes of this uncontrolled proliferation... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...discovering and designing molecules that are showing some promise in their ability to inhibit Ras. Stephen...

...with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol for their favorite...research community if you've had success with it. Some great places to get your results out there include...advancing rapidly and it wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease...

...the protein's behavior in cells, in solution or something in between, you are using a tag with a serious..., 4]. Solutions to the polymer problem There are some simple solutions available for researchers to deal... equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers describing the...

...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...microhomologies.MMEJ has certain advantages over HDR. Some species don’t have good HDR systems, and NHEJ will...amplification. Given the inefficiency of HDR for knock-ins, some labs have used NHEJ for whole plasmid integration...

...! In this blog post we’ll provide an overview of some CRISPR mammalian expression systems, the typical...includes several different methods, so we've summarized some key features of common  delivery methods below: ... in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency of...

...computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns out over time...engineering qualities, what these parts allow is for someone to forward engineer a system of 10 genes. You can...can now forward engineer a 10-gene system to do something. We know from our testing of these parts that ...

...has been a powerful tool for quantifying AAV for some time now. When titrating AAV by qPCR, viral DNA ... template control (NTC) well is common. Here are some steps you can take to achieve a clean NTC: Have...is also being used in the detection of microbes. Some applications include measuring viable probiotics...

...or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three (Servellita...types (Tatsis et al., 2007), potentially because some of the wild-type viruses can persist as a latent...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

...though exogenous DNA can be used as well. Below are some considerations for designing donor DNA for successful...shorter homology tracts have recently been used with some success (Yu et al., Nat Chem Biol). Mutate the ...HDR-promoted cycle longer. This approach may have some negative outcomes on viability if cells are arrested...

...cell biologists talk to chemists? One reason is something called “the photon budget”: each sample has a ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing...where washing is difficult, such as intact tissue. Some chemical labeling strategies such as the bisarsenical...

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...to analyze product size. This blog post discusses some of the key things to consider when performing colony...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

...versatile protein to suit their experimental needs, and some of the common applications in the lab. Why Green...instead related to the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...as the gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in ...