We narrowed to 583 results for: BASE;

...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as...

... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...

...populations.   Figure 2: Compensation example based on experimental sample. Flow plots showing a sample...

...PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system...

...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...

...journal clubs have become popular, including those based on Twitter. At my former academic department, we...

...acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in...

...vector delivery is often used, the CRISPR approach based on HDR has the advantage of being a potentially ...

...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ...

...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent...

...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https...

...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  ...

...find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying...

...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid.  The Addgene Vector Database has more information about common vector backbones...

...Hopefully we will have new tools for plant science based around those technologies in the near future. Science...

...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online...

...could be harnessed or enhanced for future MagLOV-based tools, just as light-induced conformational changes...

...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection...

...application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead...

...you do? What follows are just a few suggestions based on what I have found to be especially beneficial...

...CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool...

...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

...over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The...

...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...

...followed by sequencing and further data analysis. Based on Figure 31.5.2 from McDade et al., 2016. ...

...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for...

...appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...

...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

...together a really great solution that covered all the bases. You may have also used your writing skills to convey...

...resources towards the development of a new therapy based on its market need and anticipated commercial success...

... 1a). Other strategies that have been developed based off of this concept include: Self-labeling tags...

...Ohashi, T., et al., An experimental study of GFP-based FRET, with application to intrinsically unstructured...

...whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before...

... it, could allow idea generation and extension, based on existing, published data. The importance of data...

... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...