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We narrowed to 866 results for: FER

Showing: 481 - 520 of 866 results

  1. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...hiring a scientist. Of course, we have different criteria for different roles but for almost every position...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get ...

  2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound...40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...

  3. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...paper is much more detailed. I prefer to do it on another day - preferably the following morning - as usually... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, ...

  4. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...

  5. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...this emerging technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., ...obvious next applications are to expand the tool to different brain-organ axes. Alternatively, the system could...Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology...

  6. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...for and in HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... together as useful and productive, forming a different constellation of interests and skills than what... experimental design and data with authors and referees. And I am constantly teaching – often explaining...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...short program to build skills and connections. AAAS offers a summer program that gives graduate students an...

  8. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes...viability question. The miLab you see today is far different than what was originally planned, due in large...the potential contacts your personal network can offer, you are really on your own to build a strategic...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible...

  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...specificity of their luciferase assay, they concluded that components in the buffer used for protein purification...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...

  10. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...Scotland, who had just the right infrastructure and offered to collaborate with them. The partnership was fruitful...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...

  11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ... dynamic nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature...

  12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...intended to help early career scientists attend a conference of their choice to share their fluorescent protein...and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach: Developing...and temperature and leaf autofluorescence can interfere with microscopy. Thus, Bernach sought to develop... Jennifer Tsang...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...Sequencing and QC at Addgene.  References and resources References Mosch, R., and Guchelaar, H.-J. (... T-cells or monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through...vitro approaches enables researchers to develop safer and more effective mAb therapeutics. The in silico...

  14. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...

  15. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...side of caution when adding buffer back to the solution. Add a bit less buffer than you would to achieve...process of generating viral particles. Limit tube transfer - the fewer opportunities your viral particles...working on methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant...

  16. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...tend to be more "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing...presents regularly with labs around the world. You may prefer a more competitive style to spur you to succeed..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...

  18. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...abhiaggarwal.bsky.social‬).   References and Resources References Aggarwal, A, et al. (2025). A sensitive...are used simultaneously to monitor or control different cell types or signals in the brain. For this to... to work, each indicator needs to emit a different color of light and be compatible with the lasers available...

  19. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very first professional... who is my audience, and what do I want to be different for them when I’m done? What are we trying to ...    Here, this image is being used to convey the difference between a gradient tube before and after ultracentrifugation... For instance, you would present information differently in a poster compared to an online abstract or...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...further your efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services and even...

  21. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...consumer-grade components. In this field, often referred to as 'frugal science', existing components are...problem-solving platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper...microscope. Lower left panel shows an image of differentiating monocyte with planes and hardware to it. Right...image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling...

  22. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...Sanders of ComSciCon, the Communicating Science Conference series for graduate students. I believe that...giving a public talk, ask a friend who works in a different field to listen to you practice.  Ask them what...addition to this annual event, graduate students in different regional and disciplinary communities gather at...here: https://comscicon.com/. All our events are offered free of charge to accepted students, and we pay...

  23. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...found in genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular...signaling pathways as part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are...cancer mutant collection and the genetic pathway reference collections are described in this paper Systematic...Central PMCID: PMC5386591.  The genetic pathway reference collection is described in this publication ...

  24. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ..., without the FMO, it would be difficult to differentiate the Ly6C negative population from the Ly6C positive...like for a particular marker, you can use the reference plots provided by the antibody companies or check... use the APC IgG2c isotype control). The only difference is that the isotype antibody will not target ... as to when you need isotype controls. Our lab prefers to use them to gate our populations, but others...

  25. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly, NHEJ has a vast toolbox...from the broken end after cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other...Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM...Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens...

  26. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...widely utilized across many different cell types and each one requires different elements for vector propagation... features of yeast vectors and explain how they differ from the bacterial elements previously described...auxotrophic markers and includes the associated references.  Yeast selection marker What does the marker...

  27. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...stem cells edited to carry mutant versions of 15 different disease-associated genes. Addgene spoke to Musunuru...mutations. Once that's done in stem cells, we can differentiate those mutant and wild type stem cells into any...all have the mutations but they will also have different genetic backgrounds. It becomes more of an apples-to-oranges...suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system...

  28. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...ultracentrifuge.  Figure 1: An outline of the different steps in RNA extraction. Researchers at the... Reagent®, RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... ensure DNA contamination is not an issue.  References                 Allewell NM, Sama A (1974) The...

  29. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...negative) the action potential of a cell, the more differentiated and quiescent it is, and the more depolarized... toggled between open and closed states using different wavelength light pulses. Other resources from...bioelectricity, bioelectric researchers in many different fields came together to found the journal Bioelectricity...regeneration, medicine, and synthetic bioengineering. References Chernet, Brook T., et al. "Use of genetically...

  30. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... Jennifer Tsang...

  31. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for...2013, which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...

  32. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... for your specific scenario.    References and resources References Bates A, Power CA (2019) David vs...is relatively easy to physically divide up the different regions, both through genetic and proteolytic ...broken up, there are a few commonly available different fragments that retain antigen binding (Figure ...

  33. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... disease, and much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et ... dsDNA viruses yield dsRNA through a slightly different mechanism thought to be associated with bidirectional...blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  34. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...of trainees? Are there too few or too many at different levels? What does an ideal and productive workforce...a young scientist’s perspective, and the many different paths that biomedical graduates take in their ...policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing US biomedical...Henry, and Michael Roach. "Science PhD career preferences: levels, changes, and advisor encouragement."...

  35. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type IIS restriction...256 distinct flanking sequences, sequences that differ by only one base may result in unintended ligation...constructs, assemble standard plasmids parts in different combinations, or other new and exciting applications...constructs in a single, high-efficiency step. References: Sequential amplification of cloned DNA as tandem...

  36. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...compatible with multiple FP(11) fragments, even from a different base FP (for example, EBFP2(1-10) can be used ... useful. Good luck, and happy experimenting! References and Resources Additional resources on the Addgene....org Fluorescent Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other...inducible genome editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved...

  37. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang... May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving...loadingExample: AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas...

  38. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...CRISPR experiment. Happy cloning! References and Resources References McGee, A. V., Liu, Y. V., Griffith...chosen because of their broad applicability in different model systems. What’s the error rate? The Doench... pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred over 512) Mammalian Lentivirus pRDA_789...

  39. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific AAV delivery...quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have been... viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nature...the Gradinaru lab, AAV1-X1, was developed by transferring the 7-mer peptide region from variable region...

  40. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...and epigenetics. So, I sought out a number of different opportunities to broaden my training beyond the...major part of our everyday work is to liaise with different organizations and individuals, to identify ways...collaborating, and to organize events that bring different voices together to talk about genetics. In addition... such as scientific, educational or industry conferences, as well as schools, community events, churches...
Showing: 481 - 520 of 866 results