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We narrowed to 839 results for: LEA

Showing: 481 - 520 of 839 results

  1. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI, it’s easy to miss...Starting with a recap of accomplishments, from learning new techniques to finally figuring out a tough...the yearly planning meeting is that the trainee leads the conversation. As the conversation proceeds through...

  2. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...and easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...on these and other tags used in the pCri system, please see Plasmids 101: Protein Tags. Beyond E. coli...purification. For a more thorough introduction to pCri, please see the original publication cited below. pCri ...

  3. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...some Addgenies. As the gala came to an end, it was clear that the Addgenies had no intention of ending the...tough but memorable one. At the gala, one thing was clear: the sense of family is still the backbone [ba-dum-chh...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

  5. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit that controls...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

  6. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...materials from the Addgene repository. Read on to learn more.  What will be available?  Over 3000 AAV plasmids...AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering process will look slightly... the identity of the packaged DNA and serotype. Please note that Addgene cannot perform functional or ...

  7. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading...the IBEX Knowledge-Base is named on the Zenodo release in keeping with their value of shared ownership...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, ...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

  10. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...those collaborations over the years, he and his colleagues built a Molecular Expressions collection including...much easier for scientists everywhere to get a clearer picture of that intracellular world, even as the...promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson...

  11. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ... positive selection screening. They and their colleagues including Feng Zhang used the GeCKO library to...CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...The guides are optimized to maximize on-target cleavage and minimize off-target effects.   New from ...

  14. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If the pathogen...antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation.  Antibody-dependent... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

  15. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch... cited. If the information seems incomplete or unclear, Bairoch follow up via email to the authors of ... you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

  17. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...require the use of specific sequences. Read on to learn how easy this process is and to get tips for applying...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When ...

  18. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...Product Development Manager at eScience Labs. To learn how Caitlin got into scicomm and landed her first...series! Additional Resources on the Addgene Blog Learn more about alternative science careers here Interested... for scientists here Resources on Addgene.org Learn more about careers at Addgene ...

  19. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...asking what approach will I take and what can I learn, learning the difference between good and bad data, and...establishes your professional brand. It offers colleagues insight into your interests and will determine...

  20. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...hope that this provides a clear overview of what you need to submit data. Please note that the data hub ...generated with a caption. For microscopy images, please include channel imaged, a color legend for probes...

  21. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ... nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics researcher on...condensed into two discernible paths. The first leads into the world of industry. Along this road, science...Understanding just how the public utilizes insights gleaned from scientific research is, after all, one of ...

  22. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ... A diagnostic digest is one of the easiest and clearest ways to determine if your sample has recombined...extraction to isolate the full, intact plasmid. Please see our gel extraction protocol for more details...more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for...

  23. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...antibodies are supplied with no sequence information, leading to problems with reproducibility and an inability...to polyclonal or monoclonal antibodies. You can learn more about recombinant antibodies in this blog post...Addgene blog Check out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal ...

  24. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...suggesting that outcomes are already determined by at least day 13. However, distinct transcriptional states...335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS...delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study ...

  25. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast...technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al...used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the...

  26. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...expertise you’d like to contribute to the blog, please email us and we’ll get you writing!     www.addgene.org... Service or any of the materials we distribute, please send an email to [email protected]. Your questions...

  27. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  28. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent...Plasmids 101 2nd ed, the 3rd edition has now been released), and have started providing recorded interviews...Resources at Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository ...

  29. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology...on the first attempt for 6/7 genes. The data are clear - the SEC-based system is both simple to use and...rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution!   References...

  30. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein,... also points out that the approach he and his colleagues took to optimize sensitivities can now be applied...calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised self-associating...

  31. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal...widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific...and follow the DNA sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires...

  32. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...how the shared reagents should be used. Setting clear expectations from the beginning makes the sharing...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...

  33. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease onset, but ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something....2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the ...

  34. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...for reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number...Bauer (Addgene plasmid # 87155 RRID:Addgene_87155)” Please note that we only provide recommendations for how...Check out this post on Scientific Reproducibility Learn about the anatomy of a plasmid page at Addgene ...

  35. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George Church...3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice on staffing... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

  36. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...immune response. There are three main pathogen clearing functions or effector functions of the constant...are two IgA subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies 101...NBK27162/ Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

  37. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... specificity of monoclonal antibodies, they also lead to more reproducible experimental results. When ...NBK100203/ Additional resource on the Addgene blog Learn more about research applications for antibodies ...Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

  38. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing... small enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the...stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR...

  39. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...our Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one..., McPhee DA, Verity E, Dyer WB, Wesselingh SL, Learmont J, Sullivan JS, Roche M, Zaunders JJ, Gabuzda ...Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral...

  40. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...This is just a primer so that you are aware of at least some of the possibilities. Basically, if you can...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince...PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome ...
Showing: 481 - 520 of 839 results