Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 796 results for: arc

Showing: 601 - 650 of 796 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

  3. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...

  5. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Alternative envelopes are powerful tools as they allow researchers to target transduction to a particular cell type...be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing...

  7. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... 16 labs around the world and can be used by researchers to validate their qPCR assay (Lock et al., 2010...titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed PMID: 23828206. PubMed Central...

  8. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...work for biotechnology companies and help other researchers use the company’s products successfully. In technical...involve interacting with other departments such as research and manufacturing, quality assurance, product ...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in bacteriology and... Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research microbiologist specializing in bacteriology and...

  10. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...thought it would be a good idea. After a bit of research, we realized that the r/Science AMA series was...posts were about toxic advisors, fruitless job searches, and even despair at having chosen an academic...

  11. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...→ office culture shock? Working in an academic research lab involves a few key features that can be difficult...community (and sometimes conduct their own independent research!). There are also organizations like Science from...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...pathways and spontaneous mutations.  Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant...

  14. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...target!) and use them in numerous fields including research and development, diagnostics, drug discovery, ...interfere with conjugates. Make sure to do your research before conjugating at the bench.   Indirect ELISAs...

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus spp., Thiobacillus...enteritidis, S. typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas ...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...A recent survey of PhDs found that many researchers feel they lack formal training in a variety of transferable...'re helping to fill this gap by both helping researchers recognize their MANY transferable skills learned...

  17. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...pathogen more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein...Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...equipment and the long turnaround times, have led researchers to turn to other technologies like CRISPR. ...Learn more about plasmids available for COVID-19 research Resources on Addgene.org Get the basics about...

  22. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...permeabilized membrane to enter cells. Depending on the research question and downstream application, one or several...depending on the application. Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard...

  23. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...used for virtually all kinds of experiments and research fields, for any experiment, your first three gates...visualize four populations at once.   Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard...

  24. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...mice under a variety of behaviors, opening up new research directions that were inaccessible with earlier...hope these tools will be useful in your future research, and we’re looking forward to even more innovation...

  25. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...sequence trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org...template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org...

  26. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...we decided to write this quick guide to help researchers choose the probe for their needs. You’ll find...biosensors, and optogenetic tools. Follow her recent research @daria_gift_shch.     References and resources...

  27. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...Blogger Kristian Laursen! Kristian Laursen is a researcher at Cornell University.       References 1. Breslauer... for site-directed mutagenesis." Nucleic acids research (2014): gku1189. PubMed PMID: 25399421. PubMed...

  28. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...of E. coli currently available – a quick Google search suggests there are hundreds. This only includes... of B strain is a bit more convoluted due to researchers sharing and renaming samples throughout history...

  29. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https://doi.org...mouse and macaque cerebral cortex. Neuroscience Research, 93, 144–157. https://doi.org/10.1016/j.neures...

  30. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...A recent survey of PhDs found that many researchers feel that they lack formal training in a variety ...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...

  32. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...amounts of pure product in an affordable way, researchers test many different combinations of promoters...testing CRISPR tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other...

  34. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...tightly to DNA even after cleaving it, so many researchers think the enzyme needs cellular recycling factors...valuable laboratory tool are still an area of active research. New details are still being uncovered, and different...

  35. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...sample=control Gentle, et. al No Raw Data researcher defines log-linear phase Liu and Saint No...Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752...

  37. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...Blot Doctor page, or robust discussions, like ResearchGate’s western blot forum.  Blocking Don’t skimp on...dots, lines, smudges, etc...) Technical errors Search online/ask an expert Review protocol videos...

  38. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...(IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated and...

  40. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...2022! Share your AAV data, connect with other researchers working with AAV, support open science, and maybe...

  42. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...Updated March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name...

  45. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...able to support rapid progress in all areas of research. We welcome your comments... Where do you think...

  47. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... provide a brief description of the applicable research project when purchasing export-controlled materials...

  48. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...: Guest Contributors of 2023 8 Great Depositor Research Stories Interested in contributing to the Addgene...

  49. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... shared by 草方 (@gracie_fang) That’s right, researchers of the world. Cap your Eppendorf tubes, hang ...

  50. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...sciences and techniques that simplify and expedite research. We recognize, however, that we can’t do it all...
Showing: 601 - 650 of 796 results