We narrowed to 329 results for: AGA
-
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...
-
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....University in Canada. He's happy to be contributing again to science. Acknowledgements Chan Zuckerberg ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m.... to prevent any non-recombinant vectors from propagating. The ligation-independent methods like Gibson... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m.... Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....price point, which should be carefully balanced against the limitations of the assay. Fig.... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....PCR product is often visualized on a diagnostic agarose gel to confirm product presence, size, and relative... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....most popular with our requestors. However, we can again see quick adaptation and steady use of all types... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....action potentials which are electrical charges propagated through multiple cells to do things like translate... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....colony phenotype upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m.... and development before it can truly be pitted against CRISPR head-to-head. Exciting features of NgAgo... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....result – blastx checks all six possible frames against the database. Another benefit is that a frameshift... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....other words, these parts can be used over and over again in combination with novel genes to get very precise... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....D-F) High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...