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We narrowed to 514 results for: CIT

Showing: 241 - 260 of 514 results
  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express... Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new eBook...
  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...where limited expression of Cas9 is required and specificity is a concern, such as knockout generation or ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...
  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...efficiencies of 90-100% (Fig. 2B), and >99% on-target specificity. Additionally, by expressing multiple crRNAs ...the CRISPR toolbox RNA-guided transposases are exciting new additions to the growing CRISPR toolbox for...
  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular... mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...
  5. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...of concentrations but suffers from decreased specificity, especially as it approaches the minimum and ...are more disheartening than seeing a potentially exciting result quashed by a bad standard curve. Take the...
  6. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...
  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...
  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression of AAV...
  9. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...
  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... make your ideas easier to understand and more exciting. Practice presenting your chalk talk Perfect your...research program. You should be proud of it and be excited about getting the opportunity to realize your vision...
  11. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...nonprofits, who tend to prioritize candidates that are excited about their work and mission.  How do I know what...you excel in the role. It can also convey your excitement for that particular job, your commitment to the...
  12. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...development, transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC...
  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...culture. Our developers are a diverse group of ethnicities, cultures, genders, educational, and professional...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...
  14. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed raising ...level. Not only is this in direct contradiction to explicit policies set up other institutions (like University...
  15. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...information (or links to information) for assessment of veracity of the conclusions drawn. The main difference ...limited view of data. In addition to enhancing veracity of collections and interpretation of data, publishing...
  16. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to... future post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type ...
  17. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to ...powerful boost to genome editing experiments. We’re excited to see how you continue to use and develop AAVs...
  18. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...described in 2013 (Cong et al, Mai et al) we were very excited to set up a CRISPR-based editing platform in our...study what’s wrong with the cells. However, our excitement turned into frustration when we sequenced our...
  19. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection than one...an efficient allelic exchange system, he is so excited to use it to explore how hospital-associated Gram-negative...
Showing: 241 - 260 of 514 results