Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 504 results for: CIT

Showing: 241 - 260 of 504 results

  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...nonprofits, who tend to prioritize candidates that are excited about their work and mission.  How do I know what...you excel in the role. It can also convey your excitement for that particular job, your commitment to the...

  2. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...development, transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression of AAV...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... make your ideas easier to understand and more exciting. Practice presenting your chalk talk Perfect your...research program. You should be proud of it and be excited about getting the opportunity to realize your vision...

  5. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...culture. Our developers are a diverse group of ethnicities, cultures, genders, educational, and professional...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

  7. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed raising ...level. Not only is this in direct contradiction to explicit policies set up other institutions (like University...

  8. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...information (or links to information) for assessment of veracity of the conclusions drawn. The main difference ...limited view of data. In addition to enhancing veracity of collections and interpretation of data, publishing...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to... future post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type ...

  10. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...and the fluorescence signal weakens. (b) The heterogenicity of proliferative behavior in a cell population...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Overall, the synthetic rAbs demonstrate remarkable specificity for their targeted integrin subtype, even when... with the Springer lab, reported the binding specificity, kinetics, and affinity of these 11 newly discovered...

  12. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Attendees will include grad students (that are solicited for feedback), possibly the Dean, and even some...decks leads to the opposite and can suffocate an exciting idea in a swamp of unnecessary details. You DO...

  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to ...powerful boost to genome editing experiments. We’re excited to see how you continue to use and develop AAVs...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or break your...

  15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...described in 2013 (Cong et al, Mai et al) we were very excited to set up a CRISPR-based editing platform in our...study what’s wrong with the cells. However, our excitement turned into frustration when we sequenced our...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection than one...an efficient allelic exchange system, he is so excited to use it to explore how hospital-associated Gram-negative...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Proteins can also be biotinylated by exploiting the specificity of the E.coli BPL, BirA. If you recall, BirA ...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b...

  18. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ... Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins...the reaction, or have difficulties with kinase specificity in vitro. The establishment of a system in which...

  19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...
Showing: 241 - 260 of 504 results