We narrowed to 402 results for: LARGE

1. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...

2. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ... advantage of this technique is the potentially large number of single clones obtained (on occasion we...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...and actual molecular size – it is possible that a large or charged FP fusion can change the subcellular ...

4. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...

5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...proteins are split between strand 10 and 11 to yield large (1–10) and small (11) fragments. In several cases...

6. PCR Applications for Copy Number Variation Assays

   Type

   Blog Post

   Published

   April 26, 2022, 1:15 p.m.

   ...amplicons.  Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ...

7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

8. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...

9. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel...

10. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...you are interested in viewing which has drawn a large crowd, making it difficult to see the actual poster...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...

11. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...

12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...relies on antibody chains, which are relatively large and are not expressed consistently throughout cells...

13. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...

14. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...

15. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...Permeabilization What is the point?  Antibodies are large proteins that need a little help crossing cell membranes...

16. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...the insert site. A positive clone will produce a larger size product then a negative clone without the ...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared...

17. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ... parts. That was the motivation for releasing a large parts kit. Once you have so many parts available...

18. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to...

19. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...

20. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...