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We narrowed to 777 results for: REP

Showing: 241 - 260 of 777 results

  1. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20... 20 additions we've made to our viral vectors repository since March.  Addgene is now offering an AAV2...gene expression! We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool...Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool...New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

  2. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ... to conduct experiments faster, skipping the preparation steps of production, purification, and titering...customizability, choosing the cargo, serotype, and prep size they need for their experiments — and we’ve...them out in record time.  However, our in-stock preps remain a popular choice thanks to the convenience...cortical interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

  3. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...express my concerns and request a replacement. I got my replacement in the end and the issue was resolved... A degree in the life sciences prepares one for numerous non-academic careers. Still, many start their...support roles. Examples are Technical Service Representative, Technical Support Specialist, Technical Support...less suitable for troubleshooting or issuing replacement products. In order to find good and helpful answers...certainly there won’t be a shortage of amusing reports where someone tries to “optimize” a particular ...job. Solutions may also be achieved by simply replacing the product in question. This can solve the problem...

  4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...jejuni (Cj) NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC...in mammalian cells. The REPAIR (RNA Editing for Programmable A to I Replacement) system fuses Cas13b to...when trying to edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....below.  Cas9 species PAM sequence ’) Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus...

  5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ... organisms) -  perhaps you’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project...used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black footed slipper...real bottleneck. New gif for #MolluscMonday: #Crepidula atrasolea juvenile, light microscope with polarizing...it in a fertilized egg to get edited embryos (replacing the nucleus is still very difficult). Cuscuta ...targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming with CRISPR/...full hydra and hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the...adult female mosquitoes. Now they are trying to replace embryo injection (hard to do, expensive, not every...

  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...different CGBE tools allow the researcher to direct the repair of the abasic site. But what if an editor could...excision is the most common type of base excision repair. But mammals also have an enzyme to remove inosine...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse... or to create the substrate for a base excision repair pathway. The team behind AYBEv3 introduced the ... the non-edited strand to encourage the cell to repair the DNA using the edited strand as a template —...editors Excises G, T, or C, respectively, to be replaced with another base. References Chen, L., Hong...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...gradients? Excellent! Now, let’s head west, intrepid reader! Protein Prep The first step in any western blot is...minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are the wrong size on your gel...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...antibody’s dynamic range. A) The protein dilutions, representing expected concentrations, are all within the ...antibody’s dynamic range. B) The protein dilutions, representing expected concentrations, are outside of the ...blot, you may want to consider using a different reporter or, if you don’t need to compare the protein amounts... amounts directly, stripping and reprobing. Just make sure to go from least to most abundant protein if...

  8. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...reference: OpenWetWare Preparing chemically competent cells (protocol)  Preparing electrocompetent cells... Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...Research to make competent cells because cells prepared using this kit can be transformed without heat...of electrocompetent cells (protocol)  Cells prepared using the Z-competent TM buffer can be easily ...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...fluorophore-conjugated streptavidin protein, which binds the primary antibody through the biotin-streptavidin interaction...detection. The advantage of this system is that streptavidin conjugates are commercially available for a ...fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!   ... two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody host species interactions...include transcription factors, histones, and DNA repair enzymes. Note that when using the nuclear fix/perm...or protein-protein interactions, like biotin-streptavidin binding and antibody host species reactivity...

  10. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...talk to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ...for poor quality slides with sufficient time to prepare (and it does take time). For important talks, ask...loves a great animation. 8. Predict questions, prepare answers Speaking pros always have a few extra slides...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...their phones. Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than...when I then ask “and who has deposited to the repository?” When most or all of the hands go down it is...

  11. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It’s...interested in getting started? Reply in the comments below or tweet us your reply & #scientistswhotweet. And...users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines on Twitter. Twitter users...Follower Network of Scientists on Twitter. Each dot represents a scientist. Only scientists who follow each ...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...engineered not to cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA...cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization of the Cas...will generate a ssDNA break which can easily be repaired by cells with a near zero likelihood of mutation...insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific detection... these approaches is that NHEJ must perform the repair to allow for detection, not Homologous Recombination...non-cutters If your Cas experiment involves activation/repression or any other effect which does not depend on ... be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify...

  13. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ... ReFigure. Reading and exploration including replications and experiments resulting in “negative data”...performance as in this antibody ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium...reagent performs well, or you might publish a replication experiment that you conducted as part of setting...traditional publications. By submitting your data to a repository (Figshare is currently supported by ReFigure ... specialized knowledge of which findings are reproducible. For an example, see our ReFigures on the therapeutic... interim research products (pre-registration, preprints, data, software, micropublications) in grant proposals...

  14. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu...adapted from Govorunova et al. 2022.     HcKCRs represent a long-sought tool for researchers to control ...s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org/10.1101/2021.09.17.460684.)   ...

  15. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository for ... Calculator app for iPhone Reagent and Data Repositories These links can help you find experimental and...for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX...IBEX Knowledge Base: A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies...human, and marmoset brain IHC.  FPbase: A data repository for fluorescent proteins and their properties...

  16. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that...published work meets the accepted standards of reporting in the discipline and reduces the dissemination...information that would prevent the study to be replicated or omission from the literature. I think that...data on ... The language is ... The general representation of data is... The figures are ... In general...the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare, Triad) while ...

  17. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...efficiency. If this finding is replicated in larger studies, it will represent a key advantage over Cas9, ... RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO REPEAT THE RESULTS DISCUSSED BELOW Biologists are going...the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight...NgAgo is also able to mediate homology-directed repair when a template is supplied. Find Plasmids from..., this property could be an advantage. However, repair after base excision would alter the target sequence...

  18. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...become something tangible.  When you think of entrepreneurship in the 21st century, who do you think of?  ...server space. But what if you want to be an entrepreneur in the health sciences?  Is this a different...with innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into...lessons I learned while becoming a health science entrepreneur at 22. Start with your network You've spent ...leave people remembering your potential as an entrepreneur, not your credentials. If you have an idea, ...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ... you the benefit of a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason...observed high fluorescence resilience to these preparations. After crystalizing the hfYFP structure, the...

  20. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ... and localization in vivo. The LipoGlo system represents a new, intriguing approach to expand our understanding...cells. These changes were larger than previously reported for other organisms, and the Gottschalk group ...
Showing: 241 - 260 of 777 results