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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 580 results for: RON

Showing: 241 - 260 of 580 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...protein in the lane, your loading control won’t be strongly influenced by unexpected variations in any individual...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should be widely applicable...marking cell types in the nervous system By Ashley Waldron The mammalian nervous system is composed of myriad...

  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...and Ruddock., 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing ...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...and screened with in vitro assays to select for strong binders. Then the sequences for the variable domains...

  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...Jonathan. "RNA fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908...

  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...plasma HIV-1, they are at increased risk for other chronic diseases, including dementia, gut disorders, nerve...disease risk is attributed to latent HIV reservoirs, chronic inflammation, and negative metabolic effects of...in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed... fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance...not degraded, cell cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq...

  8. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...substrate generates a proportionate luminescent signal, strong enough to detect from proteins expressed at their...endogenous levels. HiBiT is compatible with both strong and weak promoters, and even modest endogenous ...compatible with both intra- and extracellular environments, making it effective for analyzing real-time...

  9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...confidence that they aren’t simply doing an experiment wrong. However, even simple procedures like pouring a ... easier to learn new experimental techniques. Electronic lab notebooks also play a role here by making...scientists with positive results have done nothing wrong, but the problem is that only these positive results...

  10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...from Yang et al. requires two gRNAs targeting two intronic regions and two repair templates in order to insert... allele requires you to target 2 loxP sites to intronic regions 0.5 - 0.8 kb apart. In the example below...CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer ...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... was engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293...genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed ...

  13. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...

  14. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your talent...person makes time for you, as needed, this is a strong indicator that this person cares about your science... to finding out you've been doing something all wrong or that a reagent has not been convincingly demonstrated...

  15. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...co-workers who want to make our organization as environmentally friendly as possible. We worked with Andrew... unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...easier. Trust Ultimately, your team could have a strong sense of purpose, an excellent leader, and perfectly...

  16. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...carrier of genetic information and led to the coronation of DNA as the molecule of life.  This was further...genetic engineering experiment which goes horribly wrong. The plot of the movie involves The Rock and his....111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. Science...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop...list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors, absolute FRET acceptor...

  19. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ...

  20. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...around the world!) that have kept Addgene at the forefront of life sciences research for the past decade....not just for a better day at the office but for stronger science as well. This blog is our way of continuing...
Showing: 241 - 260 of 580 results