...confidence that they aren’t simply doing an experiment wrong. However, even simple procedures like pouring a ... easier to learn new experimental techniques. Electronic lab notebooks also play a role here by making...scientists with positive results have done nothing wrong, but the problem is that only these positive results...

...from Yang et al. requires two gRNAs targeting two intronic regions and two repair templates in order to insert... allele requires you to target 2 loxP sites to intronic regions 0.5 - 0.8 kb apart. In the example below...CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer ...

... was engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128...

... E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293...genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed ...

...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...

...carrier of genetic information and led to the coronation of DNA as the molecule of life.  This was further...genetic engineering experiment which goes horribly wrong. The plot of the movie involves The Rock and his....111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. Science...

...e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your talent...person makes time for you, as needed, this is a strong indicator that this person cares about your science... to finding out you've been doing something all wrong or that a reagent has not been convincingly demonstrated...

...co-workers who want to make our organization as environmentally friendly as possible. We worked with Andrew... unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...easier. Trust Ultimately, your team could have a strong sense of purpose, an excellent leader, and perfectly...

...and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop...list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors, absolute FRET acceptor...

...pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for...are used in the lab? Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools ...

...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ...

...around the world!) that have kept Addgene at the forefront of life sciences research for the past decade....not just for a better day at the office but for stronger science as well. This blog is our way of continuing...

...spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years...cell type, in more complex and physiological microenvironments. These screens have primarily been performed...

...developing a strong graduate student community and the need for belonging in the scientific environment 3:09 ...

...spatially with Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

...they have a graduate degree in science. I find it ironic that we do all of our training as scientists (5...jobs (that is what networking is about), but be upfront about interest in a job. Ask openly to be connected...

...activity and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation...

...liberated to adorn the walls of universities and the front pages of newspapers. Science is now being adapted...while - a tool to expand perception, which is ironically what science is about. For me it is essential...

...increasingly accessing websites other than through the ‘front door,’ the homepage is still important. It needs...easily. In the redesign, the new search bar is now front and center, with a clear call to action - Search...

...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...