We narrowed to 771 results for: SOM

...Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for some quick advice and resources for hiring research staff... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...The Howard Hughes Medical Institute (HHMI) offers some great resources for Early Career Scientists, including...

... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ... rare variants that haven’t been characterized? Some have developed exciting computational algorithms...acid change based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often...genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, et al. Cancer...

...myself would take less time than the seemingly burdensome task of explaining the steps to a lab assistant...lab scientist, I worked with a diverse team where some members thrived on independence, while others required...dynamics. I experienced this firsthand when it took me some time to adapt to the communication style of a new...do this while changing industries and positions. Some people discover they truly enjoy management and ...

...that didn’t get answered live - but he kindly took some time to address all your unanswered questions via...be oversized even before considering the need for some manner of polyA. In this scenario, you may be best...adopting ddPCR, which does not a standard curve and somewhat more reliable quantitation. Differences in sample...VAST) experiments using PHP.eB in the brain give some insight into codelivery efficiency at different ...

...manufacturer, lot number, and expiration date somewhere you can easily check it whenever you use the antibody...freeze-thaw cycles, as they’ll degrade your antibody.  At some point in your long and illustrious lab career, you... with other chemicals and biological structures, some of which you may need in your experiment.  If you... to buffers, can bind to amine groups present in some conjugates, blocking the conjugation reaction. Many...

... all the Cas enzymes you know and love, and even some you’ve likely never heard of. Each entry displays...adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays these requirements ... orientation relative to the gRNA (5’ or 3’). In some cases, there is no requirement, which is also documented...CasPEDIA.   Helpful resources and information For some researchers, the previous information may be all...

...set up these systems. Components of a biosensor Some of the biosensors used in research are very sophisticated...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...in multiple species, and in cell culture models. Some of them have even been expanded for multiplexed ...and safety tips. Still worried? We make virus with some of our most popular biosensors already packaged ...

...expensive because they require particular enzymes, some of which are proprietary. These methods also rely...bacteria do the work If you’ve conducted SDM using some of these methods, chances are you’d like to find... containing the mutations, the primers also have some homology to each other; ~17 bases is optimal. After...a whopping 144 kb from a bacterial artificial chromosome in just one reaction. Using other methods, such...

...month formal program. After the formal program ends, some 50% of these groups continue to meet regularly, ...development plans. However, they find it awkward to ask someone for the time commitment of a formal mentoring relationship...stage, they have a lot to learn from each other. Some have taken my advice and I recently heard from peer...organizations as it brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar...

...so-called “small molecule degraders” recruit the proteasome to destroy targeted proteins. We previously developed...polyubiquitination of the target protein and proteasomal degradation. Remarkably, small molecule degraders...dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly...between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading...

...itself: Ma et al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic...term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, ...seems impossible with current tools, thus quieting some fears about the creation of “designer babies.” The... a ‘magic bullet’: its use will always come with some uncertainty. As biologists equipped to understand...

...are encoded by single stranded RNA (ssRNA), while some exist as dsRNA. Still others use dsDNA to store ...know the sequence of a virus, you could introduce some of its dsRNA intermediates into a healthy individual...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. These ...

...ones to try?  Read on and we will provide you with some advice on the antibody selection process. Antibodies... is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated...used in a publication. Talk with your peers. If someone in your field has used the antibody before you ...peace of mind as you make your purchase but also someone to turn to for advice if you need to troubleshoot...

...as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies...much easier said than done! Each technique, and sometimes each individual assay, will need to be developed...attaching to a single primary antibody, it is sometimes assumed that the direct method is more quantitative...improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary antibody...

...like light or color. In this post, we’ll go over some of the common conjugates you may encounter and how...conjugates in an earlier post, where you can find some additional tips for working with these tools.          ...chemistry  Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This chemical...up to 80 per antibody (Mueller, et al., 1988). If some of those lysines are in the antigen binding region...

...panicked silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in...options were to stop talking until I could present something coherent or to quickly string together all the...noteworthy and memorable and awful. So try to keep some perspective. Most people won't notice, or won't ... above to make a strategy that can help. Even something as simple as telling yourself, “I can stop and...

...needs to be empirically determined. In addition, some metal ion substrates, such as those containing Co2...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...not disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions... Introduction to Antibodies.) Unfortunately, for some proteins there just aren’t any good antibodies available...

... structure? Helping to answer some of these questions, and posing some of their own are the following....   The news is not all bad, however - there are some signs that the tides may be turning. In 2013, the...- this time with the aim of finding solutions to some of the problems that were identified last year.  ...

...learn about the tools available for bacteria and some of the applications for which they’ve been used....Different Cas orthologues and subtypes function somewhat differently and can be more effective targeting...and CRISPRa using RNAP-Omega-dCas9 fusions. Here’s some others. SoxS based CRISPRa In the search for a CRISPR...Cytidine and adenine base editing in Streptomyces Some of the most industrially and clinically relevant...10.1016/j.cell.2013.02.022 Ronda C, Pedersen LE, Sommer MOA, Nielsen AT (2016) CRMAGE: CRISPR Optimized...

... measuring locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress...adult animals, and not targeted to a specific area. Some other technical considerations for stereotaxic injections...via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can...depression. In upcoming blog posts, we’ll discuss some of these behavioral studies in detail and share ...