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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 281 results for: hal.1

Showing: 241 - 260 of 281 results

  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news for...of science, communication isn't easy.  But the challenge is worthwhile and rewarding. A truly passionate...

  2. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...into account, the digest pattern depicted in Lane 1 would be expected for a XbaI/NotI digest. The program...plasmids. It turns out that restriction enzymes are one half of naturally occurring restriction modification ...

  3. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...Networking 101-Building Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the...current position Understand your interpersonal challenges if they know some of the people you work with...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...

  7. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...then go to grad school. However, about a year and a half into my role, I realized that actually, I didn’t...

  8. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...efficiently with adenovirus and AAV.   Table 1: Types of viruses and their features Choosing ...However, the cargo capacity of AAV is ~4.7 kb, almost half that of lenti and gamma-retro viruses. This is large...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...accessible to practically everyone.     Fig. 1: Open source hardware builds   Since I started...hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  11. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...medRxiv.org) in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating...Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (2022) A guide to preprinting for...

  12. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...with each protein of interest.    Figure 1: The direct approach (left) versus the indirect approach...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

  13. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. ...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...

  14. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human... up with it. The peer review process has been challenged with many closely spaced CRISPR software submissions...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...for RaPID is an RNA motif of interest. See figure 1 below for details on the key components of RaPID. ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

  16. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... an entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’re more ...

  17. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... Science Find the SEND plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA...cavity of Cas12f. The CasMINI system is less than half the size of the Cas9 or Cas12a based systems, making...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...strand synthesis. In contrast, a scAAV vector with half the size of the ssAAV genome has a mutation in the...

  19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ... yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017)....bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical...Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology 233.39 (1971): 149...opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins, the first two found...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...release for more information. References Abdallah, Khaled, et al. "Adeno-associated virus 2/9 delivery of...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...collaborations, keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...research-intensive careers. In addition, this skill is a hallmark for jobs focusing on: Science Policy, Business...
Showing: 241 - 260 of 281 results