Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 367 results for: his

Showing: 241 - 260 of 367 results

  1. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

  2. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation in live cells....

  6. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular biology research...research, chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...applications for validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ...

  9. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...

  10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...

  12. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction...of that particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation...

  13. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

  14. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

  16. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation...victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains a mixed population...

  17. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  18. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry, and flow cytometry...

  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...
Showing: 241 - 260 of 367 results