...REPAIR (RNA editing for programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated ...they confirmed A->I editing and found that 50 nt spacers increase editing efficiency but also increase off-target...

...your science, one of the big questions you'll be faced with is "how?" How are you going convey your information...learned (somewhat successfully) how to text or use Facebook. However you choose to communicate your science...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

...AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed...pediatric population. Once ~400 cases had amassed in places around the world, hypotheses began to develop that...AdV genome in them, including the E1 region). The space in the viral particle’s capacity that is made available... G. R. (2015). Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6)...

...commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as a form of “...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...sequence for Cas9, is located at the 3’ end of the spacer sequence and consists of a single A, U, or C base...each DNA target have high sequence identity to the spacer sequence and contain the appropriate PAM sequence...cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides in length and does not require...

... public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn where ...thoughts on why we decided to do an AMA in the first place and give you some reasons why you should consider...realized that the r/Science AMA series was the best place for us. We’re definitely glad we did - you can see...lot of links and content at hand in three useful places: I had posted content on many of the requested...

...vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. ...low-density lipoprotein receptors (LDLRs) on a cell surface to endocytose into the cell. The reason VSV-G is...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer... rabies glycoprotein now serves as a retrograde tracer so that scientists can identify the neurons that...2001).  This method can also allow scientists to trace a neuron’s projections back to the cell body. Rabies...

...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...mutations wouldn’t matter – the mutated copy could be replaced by simply dragging and dropping in a functional...allowing a one-size-fits-all approach to gene replacement therapy for most patients. But how does one go...chance of the insert being “dropped” in the wrong place, while increasing the number of potential applications...

...nose-poke at the active hole [1, 2].    Place preference/place aversion also models reinforcement but ...include: intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses...ideal behaviors to test reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting...hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test...

... to you: our first, second, and third place winners!  1st Place: Krampus Saves Christmas! created by the... Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team...Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by Joanne Jary from Lab ...

...activation p300 acetyltransferase dCas9 fused to the catalytic domain of p300 acetyltransferase increases levels...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification was impossible...light-regulated histone methyltransferases and deacetylases. CRISPR and epigenetics As with many TALEN-based... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...