...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...

...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...Experiment with New Careers while in Grad School Listen to Michael Koeris' Journey from Grad Student to...

...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly...

...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...

...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ...

... to the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another...

...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...

... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

...especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for ...like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing...

..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to...

... Twitter. Be ready for the interview with a long list of written questions for the PI and lab members....too, they may not have had experience writing, publishing, or discussing career opportunities with the ...

....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...

...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

.... The plasmids used to construct these RSMs are listed in the table below and can be requested through...sequences on their 5’ and 3’ ends (for insulation). A list of promoters and terminators used in our GRSM registers...

...duration of only three months and made a good job to list on my CV. Three months was a perfect duration for...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...

...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

...for obtaining a material transfer agreement, and list of vendors (such as the vector core at the University...the many primary research papers that have been published at this point.  In brief, some standard opsins...

...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in high-throughput screening...

...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...

...shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry...