We narrowed to 583 results for: BASE;

...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

...reporters have limited use in vivo because they are based on light which is very easily scattered by tissue...

...discipline are welcome. Up to this point most videos are based on research that has been published, but many discuss...

...representing ~85% of the postdoctoral workforce. Based on our communications with and research on these...

...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...

...could reveal something fascinating. Behavior is based primarily on careful observation of what an animal...

... smaller, with the full sequence being around 60 base pairs. This is much shorter than miRNA plasmids,...

... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...

...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...

...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...

...Think a university system who develops evidence-based university policy to combat sexual harassment in...

...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...

...WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations...

...interest.  Determine injection titers and volumes based on the serotype Since each serotype also exhibits...

...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools...

...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar...

...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had...

.... Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics...

...PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral analysis of fluorescent proteins...

...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...

...using the virus. You may need to adjust coordinates based on your own method of identifying bregma but it ...

... to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions...

...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

... CasPEDIA is meant to be a constantly updating database which serves as a resource for the scientific ...properties (Makarova et al). CasPEDIA is an evolving database, and all researchers are invited to contribute..., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: A Functional Classification System for Class ...

...exhaustive, so you may want to consult the REBASE database for more detailed information. Enzyme Dam...regulating genome replication, repairing mismatched basepairs or small indels that occur during DNA synthesis...

..., it should have a corresponding entry in your database. Enjoy your happy lab staff. Barcoded materials...even be worth it to have extra columns in your database to the effect of `dev_name` and `science_name`... database...

...cell lines on public databases. Image attributions (from top to bottom) Database Center for Life Science... Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...

... through comparisons with a reference sequence database. Through a series of simulations and machine learning...identify synthetic and natural sequences in Addgene’s database. They showed a growing trend of sourcing genes...

... the locations where the enzymes will cut (in basepairs), and the total number of times they cut. Use ...nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence...

... mentoring at Mentornet.net. This is an online database of mentors you can tap for email discussions. ...who enter their information in these searchable databases are usually offering their contact information...

...New York University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University...different places have very different cultures. In Basel, I felt that the time flows much slower, like the...

...still growing our "Articles Citing This Plasmid" database - and we always appreciate any help we can get...

...could exist in nature. To do this, they mined a database of microbial genome and metagenome from the Department...

... unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

...companies need to pay to obtain them. There’s a database of labels on the My Green Lab website, but if ...

...interesting connections is your university’s alumni database. Sharing a school is a little connection that ...

...Additional Resources RCSB Protein Data Bank - a database of detailed protein structural information PDBsum...

...an appropriate liaison, but you can use alumni databases, friends, and even relatives to network your way...

... (estimated to occur at a frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental...

...diversity via the Integral Microbial Genomes (IMG) database, Harrington et al discovered a Cas9 protein from...