Addgene

What Do I Do Now? Academic v. Non-Academic Career Decisions

Type

Blog Post

Published

March 9, 2017, 3:40 p.m.

          ...Management for Scientists Series

Resources at Addgene.org

Learn About Careers at Addgene

...

10 Great Guest Posts We're Thankful For

Type

Blog Post

Published

Nov. 18, 2016, 3 p.m.

          ... section of DNA into the genome of your target organism. In this post, Chris Richardson, a postdoctoral...
        

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Type

Blog Post

Published

June 9, 2016, 2:30 p.m.

          ...Genome Engineering Posts

Additional Resources on Addgene.org

Check Out Other Argonaute Technologies from ...

Plasmid Cloning by PCR

Type

Blog Post

Published

March 29, 2016, 2:30 p.m.

          ...Tips on Verifying Your Plasmid

Resources on Addgene.org

Check out Our Plasmid Reference Pages
Deposit...

Addgene's Top 10 Blog Posts of 2015

Type

Blog Post

Published

Dec. 7, 2015, 6 p.m.

          ...your own guest posts by e-mailing us at blog@addgene.org. Thank you again for a fantastic year and happy...
        

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Type

Blog Post

Published

Oct. 29, 2015, 2:30 p.m.

          ... about the role these factors played within an organism. 

So how did plasmids get their name?

In 1952...

Donations from Addgene to Yield Answers for Rare Disease Researchers

Type

Blog Post

Published

Sept. 15, 2015, 2:30 p.m.

... Kendall Morgan...

Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Type

Blog Post

Published

March 4, 2014, 4:49 p.m.

... Kendall Morgan...

What Good Citizenship Can Do for Reproducibility in Science

Type

Blog Post

Published

Nov. 7, 2019, 2:18 p.m.

          ...about reagents, and why other publishers and organizations have various different versions of checklists...
        

New and Upcoming Viral Vectors - September 2019

Type

Blog Post

Published

Sept. 12, 2019, 1 p.m.

          ...Download our viral vectors 101 eBook

Resources on Addgene.org

Learn about Addgene's viral service
Find viral...

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

Published

Sept. 3, 2019, 1:15 p.m.

          ...new family of seven ChRs derived from marine microorganisms based on metagenomic data. MerMAIDs (Metagenomically...
        

Hot Plasmids - January 2023

Type

Blog Post

Published

Jan. 31, 2023, 2:15 p.m.

          ...microscopy. Nat Methods 19, 1612–1621 (2022). https://doi.org/10.1038/s41592-022-01660-7
 
 ...

Summer SciComm Series: A PhD in Science Communication

Type

Blog Post

Published

Aug. 23, 2022, 1:15 p.m.

          ...under which misinformation can flourish. I’ve never forgot about all the anxious, whispered questions from...
        

New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

Type

Blog Post

Published

April 7, 2022, 1:15 p.m.

          ...Fluorescent Protein Featured Topic Page

Resources at Addgene.org

Find Fluorescent Protein Biosensors
Browse All...

Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

Type

Blog Post

Published

June 1, 2021, 1:15 p.m.

          ... about external collaborations

Resources on Addgene.org

Browse Addgene's COVID-19 plasmids and resources...

Scientists Map the SARS-CoV-2-Human Interaction Network

Type

Blog Post

Published

April 9, 2020, 1:15 p.m.

          ...COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can be prioritized...
        

Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Type

Blog Post

Published

June 4, 2019, 1:02 p.m.

          ...GFP
Find microbiology blog posts

Resources on Addgene.org

Find fluorescent protein biosensors
Browse Plasmids...
        

Visualizing Genomic Loci with CRISPR-Sirius

Type

Blog Post

Published

April 18, 2019, 12:53 p.m.

          ...tools
Read all CRISPR blog posts

Resources on Addgene.org

Browse our CRISPR plasmids for visualization...

Improving Transduction Efficiency with Exosome AAVs

Type

Blog Post

Published

March 14, 2019, 1:54 p.m.

          ...Download our viral vectors 101 eBook

Resources on Addgene.org

Learn about Addgene's viral service
Find viral...

The Importance of High Titer for AAV Transductions

Type

Blog Post

Published

March 1, 2018, 2:11 p.m.

          ...Expression in Mammals

Additional Resources on Addgene.org

Find Ready-to-Use AAV Preps
Find Lab Protocols...

Learning Technical Skills at a Lab Central Workshop

Type

Blog Post

Published

Feb. 20, 2018, 2:30 p.m.

          ... about CRISPR Pooled Libraries

Resources at Addgene.org

Find pooled libraries at Addgene
Find basic ...

The Crispr Babies Saga Shows the Need for Action, Not More Delays

Type

Blog Post

Published

Dec. 20, 2018, 1:15 p.m.

          ... genome engineering blog posts

Resources on Addgene.org

Read our CRISPR guide
Find CRISPR plasmids by...

"Build Your Plasmid": The Game - Play to Teach

Type

Blog Post

Published

July 17, 2018, 12:23 p.m.

          ...Arabidopsis Biological Resource Center

Resources on Addgene.org

Find molecular biology educational resources...

Plasmids 101: NGS Quality Control for Pooled Libraries

Type

Blog Post

Published

Oct. 26, 2017, 1:59 p.m.

          ... about Pooled CRISPR Libraries

Resources on Addgene.org

Browse Pooled Libraries at Addgene

...

Visualizing Translation at the Single Molecule Level

Type

Blog Post

Published

Aug. 1, 2017, 1:15 p.m.

          ... Brightest Fluorescent Protein

Resources on Addgene.org

Find Fluorescent Protein Plasmids
Check Out ...

Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Type

Blog Post

Published

July 27, 2017, 1:11 p.m.

          ...Fluorescent Protein for Your Experiments

Resources on Addgene.org

Find Fluorescent Protein Biosensors
Browse Plasmids...

How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

Type

Blog Post

Published

June 20, 2017, 2:30 p.m.

          ...Your Lentivirus Preps

Additional Resources on Addgene.org

Check out the Viral Service
Find Virus Protocols...

Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

Published

Sept. 28, 2016, 2:35 p.m.

          ...leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer, and...
        

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Type

Blog Post

Published

March 24, 2016, 2:30 p.m.

          ...CRISPR Software for Your Needs

Resources on Addgene.org

Catch up on Your CRISPR Background with Our ...

Components of CRISPR/Cas9

Type

Blog Post

Published

Feb. 2, 2016, 5 p.m.

          ... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...
        

Synthetic Photobiology: Optogenetics for E. coli

Type

Blog Post

Published

Sept. 8, 2015, 2:30 p.m.

          ...As optogenetics turns 10 years old, it’s easy to forget that this technique isn’t limited to neuroscience...
        

Addgene Welcomes John Doench to the Advisory Board

Type

Blog Post

Published

Aug. 13, 2015, 2:30 p.m.

          ...they do and what they might be able to do and to organize it and catalogue it, I mean that’s the critical...
        

Resume Writing for Non-academic Science Careers

Type

Blog Post

Published

Dec. 18, 2014, 4:06 p.m.

          ...for Curiosity Collider (hyperlink https://curiositycollider.org/) , which she co-founded, and the Outreach...
        

Choosing Your Fluorescent Proteins for Multi-Color Imaging

Type

Blog Post

Published

Oct. 9, 2014, 3 p.m.

          ...no single fluorescent protein optimal for all organisms and that if you want the brightest signal, you...
        

Fluorescent Proteins 101: Luciferases

Type

Blog Post

Published

July 22, 2025, 1:15 p.m.

          ...Proteins 101: Introduction to FRET

Resources on Addgene.org

Luciferase collection page
Promega Plasmid Collection...

Plasmids 101: Screening Strategies Used in Plasmid Cloning

Type

Blog Post

Published

Sept. 15, 2020, 1:15 p.m.

          ...molecular biology protocols and tips

Resources on Addgene.org

Learn more about Sanger sequencing
Read the ...

The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Type

Blog Post

Published

Jan. 30, 2020, 2:15 p.m.

          ... cichlid fishes. Find David online at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.
 ...
        

Behind the scenes: Addgene’s new search engine and more

Type

Blog Post

Published

Dec. 12, 2019, 2:22 p.m.

          ... the different website navigation paths, the organization and completeness of our information, and the...
        

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

Published

Nov. 19, 2019, 2:08 p.m.

          ...nanobody toolbox for immunodetection

Resources on Addgene.org

Find nanobody expression plasmids here
Learn...

Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

Published

April 5, 2022, 1:20 p.m.

          ...101: Introduction to Antibodies
Resources on Addgene.org 
Browse Addgene's recombinant antibody plasmid...
        

Antibodies 101: ChIP

Type

Blog Post

Published

March 10, 2022, 3:25 p.m.

          ...101: Introduction to Antibodies

Resources on Addgene.org

Find plasmids encoding recombinant antibodies...

Sweating the Small Stuff: Details in the Lab

Type

Blog Post

Published

Feb. 8, 2022, 2:15 p.m.

          ...Bench setup
Small adjustments in the physical organization of your workspace and your workflow can have...
        

Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

Published

Feb. 1, 2022, 2:15 p.m.

          ...101: Introduction to Antibodies

Resources on Addgene.org

Find plasmids encoding recombinant antibodies...

Antibodies 101: Isotypes

Type

Blog Post

Published

Oct. 19, 2021, 1:15 p.m.

          ...What is an Antibody animation 

Resources on Addgene.org

Browse Addgene’s Antibodies plasmid collection...

Targeted Mutagenesis with EvolvR

Type

Blog Post

Published

Feb. 21, 2019, 1:15 p.m.

          ...Mutagenesis to mutate your plasmid

Resources on Addgene.org

Browse our CRISPR guide pages
Find bacterial...

Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Type

Blog Post

Published

Feb. 19, 2019, 1:36 p.m.

          ...
Subscribe to the Addgene blog

Resources on Addgene.org

Meet the other members of Addgene's Board of...

Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

Published

April 27, 2023, 1:15 p.m.

          ... about Addgene's Viral Service

Resources on Addgene.org

Visit Our AAV Guide Page
Find Ready-to-use Virus...

Flow Cytometry Readouts: Yes, No, and Everything in Between

Type

Blog Post

Published

Aug. 20, 2024, 1:15 p.m.

          ...Surface Labeling in Flow Cytometry
Resource on addgene.org
Addgene's Antibody Guide
rAb Affinity Purification...
        

Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

Published

Aug. 6, 2024, 1:15 p.m.

          ... and Their Use in Research
More resources on addgene.org
Addgene Protocols: General Transfection
The Addgene...
        

Antibodies 101: Flow Cytometry Controls

Type

Blog Post

Published

June 6, 2024, 1:50 p.m.

          ...Conventional vs. Spectral Flow Cytometry
Resources on Addgene.org
Addgene's Antibody Guide
Browse Addgene's Antibody...
        

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