We narrowed to 329 results for: AGA

1. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

2. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

3. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

4. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

5. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

6. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...

7. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...

8. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...

9. Plasmids 101: Colony PCR

   Type

   Blog Post

   Published

   May 12, 2016, 2:30 p.m.

   ... complete, it’s time to run the products on an agarose gel to determine their size. Make sure to use an...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...

11. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ... possible to screen libraries of bait and prey against each other at the same time in order to try and...

12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...track your samples today and never lose a sample again!   Amanda Hazen is the Laboratory Operations Manager...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...genome functions in the hope of developing drugs against intractable diseases. Currently, a number of labs...

14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial...

15. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...children, so my life will be under control.” Think again. Maybe you’ll need to care for an elderly family...

16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...

17. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the...

18. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...favor a different model. Finding evidence for or against a model is a standard scientific strategy. However...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...

20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional...