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We narrowed to 507 results for: CIT

Showing: 261 - 280 of 507 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9... in order to call that gene a hit.  This is an exciting time for functional genomics, with an ever-expanding...

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...treatments for external companies. It was really exciting to work in a larger commercial company; the pay...opportunity to work in a genetics lab. As a UK citizen, I was entitled to a Work-Travel VISA, which means...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...shown to offer high activity and unparalleled specificity in genomic targeting design flexibility. The ...cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently ...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...original enzyme, SNAP-tag and CLIP-tag show good specificity for their own ligands with minimal cross-reactivity...Protein-based tags typically provide better labeling specificity than short peptide tags like tetracysteine (Jing...

  9. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...detect specific nucleotide sequences with high specificity and quantitative accuracy and even include profiling...PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...final plasmid pages and scientists will be asked to cite this article when using your plasmids. You can submit...Deposit Spreadsheet More Data for You: Find Articles Citing Addgene Plasmids Resources on the Addgene Website...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...pattern of labeling, this is supportive of their specificity for the protein. A challenge to this approach...target with very low expression, due to induced toxicity caused by the protein of interest. HLDA workshop...

  14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...other techniques all aim to improve targeting specificity or reduce the duration of Cas9 activity.  The...recognize the same PAM and had similar targeting specificity as full-length Cas9 (flCas9). When triggered ...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...of probes/primers often offer higher allelic specificity, due to the need for the target:probe/primer ...Chemistry Background noise Probe Allelic specificity Can be multiplexed Single amplicon validation...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...cleavage site, but this system probably have better specificity. A good reference is Ran et al. (2013). As ...RNA-Guided CRISPR CAS9 for enhanced genome editing specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...transfer time. Be aware that the transfer works by electricity, so long transfer times increase the heat of ...from 1:100–1:1,000. If your antibodies come from ascites fluid, try a dilution range from 1:1,000–1:100,...

  18. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...We are excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   ...

  19. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation...
Showing: 261 - 280 of 507 results