We narrowed to 791 results for: REP

...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...become something tangible.  When you think of entrepreneurship in the 21st century, who do you think of?  ...server space. But what if you want to be an entrepreneur in the health sciences?  Is this a different...with innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into...lessons I learned while becoming a health science entrepreneur at 22. Start with your network You've spent ...leave people remembering your potential as an entrepreneur, not your credentials. If you have an idea, ...

...the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think...pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what you'll find in this...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ... you the benefit of a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason...observed high fluorescence resilience to these preparations. After crystalizing the hfYFP structure, the...

...When preparing to graduate from university, many students are confronted with the question ‘what now?...Michael Koeris' Journey from Grad Student to Entrepreneur Science careers divided by the most pertinent...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...Michael Koeris' Journey from Grad Student to Entrepreneur Learn to Network and Make the Contacts for Your...

...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ... and localization in vivo. The LipoGlo system represents a new, intriguing approach to expand our understanding...cells. These changes were larger than previously reported for other organisms, and the Gottschalk group ...

...to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754 Ravindra Kumar...Look it up in Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used...removes, not just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. ...primate. Many of the systemic capsids in our repository are deposited by the Gradinaru lab. You can visit...LY6C1) Stanton et al., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated...commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be produced...region VIII of AAV9-X1.1 to that of AAV1. AAV1-X1 reproduces AAV9-X1.1’s brain endothelial cell-specific tropism...

...a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These... a cleavage free manner. The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly... For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%, both ...using mStrawberry, localization peptides, and reporters that drive the expression of the fluorescent protein...response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman...

...Figure 2: OCaMP structure and mechanism of action. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND...is 565 nm, and 2P excitation peak is 1050 nm. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND...and yellow GECIs measured with soluble protein. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND... APs in neurons expressing OCaMP or jRGECO1a. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND...Whether you're working with cell cultures, slice preparations, or in vivo imaging, there's a version that ...cultures, zebrafish, and mouse brain, check out the preprint on bioRxiv. {% module_block module "widget_221e8fe4...

...diagram depicting transcription, or DNA repair, or replication, or any number of CRISPR applications and...methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the level...this list, that does not necessarily mean the repository does not have it. That said, if you know of a...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...

... program coordinator.   6. Virtual interview prep Prepare for your virtual interview just as you would...yourself. Be mindful that January and February represent peak season for internship applications. Thus ...that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These tips are a set of...of common sense approaches that will prepare you for the competitive landscape beyond college. They will...guarantee of success. Don’t luck into an internship, prepare the road ahead for success. Every student should...

... homology-directed repair (HDR) to correct double-strand breaks (DSBs). DSB repair by HDR facilitates ... a large cohort of genes (e.g. tagging genes representing a protein super family). To overcome these challenges...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus...without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted epitope...mutations (e.g. convert the NGG PAM to NGC in your repair template) and use homology arms of 60 -70 bp . ...temperature for 10 min. Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to ...

...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...RNA that the RNAi is designed against), RISC can repress translation and/or degrade target mRNA. These ... by bidirectional transcription or as inverted repeats. These elements are almost always epigenetically...particularly enriched in highly transcribed regions and repetitive regions, such as telomeres and centromeres. These...presumably due to their abundance of R-loops and repetitive sequence. All of the R-loops described above...

...line./ Each color represents a gRNA targeting a specific chromosome. This image represents the composite ...(GAL4) was supplied. In addition to labeling repetitive telomeric sequences, Chen et al. successfully...distance! To the authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a...previously described for transcriptional activation/repression and genome editing. Ready to make your cells...engineering for genome editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID...

...propagated it, separated it into roughly identical replicate groups, and treated these groups with the same...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...would always come from the same parent cells that represent the preexisting resistant subpopulation 2. Resistant...Resistant cells among the separate replicates would have different labels - i.e. they would stochastically... Looking at barcode abundances among multiple replicate populations, they found that similar barcodes ...populations were shared by at least one other replicate; prior to treatment, there are small (previously...

...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...the target cells, ensuring more consistent and reproducible results in calcium assays. There’s another crucial...to go. Pro tip: Vectors with ready-to-use AAV preparations are labeled with the green virus particle on...

...New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab, high school...plasmid creation process. Each module of Fragmid represents a class of components, such as your CRISPR enzyme...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...ampicillin resistance cassette and an origin of replication, which are not schematized. Sizes of fragments...always, we recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing...skip a module entirely, but instead will have to replace it with a spacer.) Each component available on ...

...specificity.  Strategy #3 (ProC): Create promoters by repeating mouse cell type specific transcription factor ...expression of the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP ...expression. Surprisingly, <1% of synthetic promoters replicated the expression specificity of the wild-type promoters...database of the microscopy images of all AAVs that reproducibly labeled retinal cells can be found here.  Applications...expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result in cells where... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors References Juettner, J...

...able to facilitate and enhance DNA repair via homology directed repair (HDR). This characteristic makes ...potentially be treated by correcting the mutation or replacing the gene. A notable example is the treatment of...reconstitution with a normally functioning T cell repertoire (1). In this example, it was possible to transduce...first discovered as a contaminant of adenovirus preparations. It is an icosahedral, non-enveloped virus that...enhances endonuclease delivery for double-strand break repair." Gene therapy 17.9 (2010): 1175-1180. PubMed PMID...

...as an NPA representative and speak the NPA’s stance. I had to be professional as I represented the organization...As the Chair of the Advocacy Committee, I had to report to the Board of Directors and liaise with the Executive...accordingly. For example, the quarterly and annual reports I had to write on behalf of the Advocacy Committee...to overcome the personal frustration and submit reports in the format requested which in the long run was... opportunities around you. You just need to be prepared to take on the challenges and be proactive and...

...should be prepared with different questions than those on the prep calls, but often the prep calls provide...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Panel discussions are becoming...nonprofit models that differed from the companies represented on the panel. This "spontaneous" participation...purpose. If possible, make sure the panelists are prepared to stay around to meet and greet audience members...

... a peer-reviewed manuscript OR a preprint. In the case of a preprint submission, we are happy to update...containing instructions on how to prepare your plasmids and a prepaid label with shipping materials to ...journal. To start this process, simply enter your preprint title or a general description of the deposit....set of entry fields to describe the next insert. Repeat as needed for more inserts. When you are finished... propagated in DH5α. For plasmids with highly repetitive sequences (which can be prone to recombination...