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Showing: 261 - 280 of 566 results

  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...important to this function. With the welcoming environment on my team and at Addgene, I felt comfortable...seemed. The transition into a traditional work environment was aided by the efforts being put in by Addgene...

  2. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

  3. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...

  4. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

  6. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...Structure and Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...splice acceptors. They require insertion into an intron of a gene, where they can be spliced into the gene...

  8. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common ...

  9. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...of input material, cells or tissue, to produce a strong enough signal over background noise. This is not...protocol, the most significant being the usage of a strong detergent to permeabilize cells rather than to ...

  10. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589... Ashley Waldron...

  11. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...that there are many ways your experiments can go wrong. Whether it be a bad fridge freezing your samples...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more...

  12. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...It is probably good news for our environment that more people are paying attention to living a “green...reagents and buffers.  However, I had very environmentally conscious friends who had me thinking about...

  13. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

  14. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...

  17. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...possible option, we place the most important controls front and center, and make specialized controls available...plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into the original...

  18. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...

  20. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag...
Showing: 261 - 280 of 566 results