We narrowed to 762 results for: SOM

...Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA... achieved high editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant... interest, its dimeric nature can interfere with some applications. Several labs have been working to ...

...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take...antibody developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing monoclonal...tag have been generated in an attempt to overcome some of Myc1-9E10’s shortcomings. Wait, 9E10 isn’t the...sensitivity (Schüchner, et al. 2020). In their study, some of the newer Myc-tag antibodies appeared to be less...

...that forms chromosomes. These long range DNA interactions are often detected with chromosome conformation...factors, can increase a gene’s transcription. Sometimes enhancers are located thousands of base pairs ...created for each genomic loci of interest. This is cumbersome and also makes it hard to compare results across...

...never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools...definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration tips...to create the iodixanol gradient layers, and has some great pointers that while help you master iodixanol...

...right Tn7 end sequences. Donor bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these...and recipient bacteria that lack an integrated chromosomal copy of Mobile-CRISPRi. For firmicute gram positive...tri-parental mating. Donor bacteria contain a chromosomal copy of the RP4 transfer machinery, which mobilizes... 1 transfer in every ~100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis...

...genome. AAV instead relies mainly on episomal expression. Episomes are circular pieces of DNA that reside...expression limitations may be too constraining for some experiments, particularly in vivo ones. In those...& Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell...

... training prior to conducting mouse experiments. Some of these techniques are what we call “beginner”,...injection into the sciatic nerve. Mice likely experience some muscle soreness after injection, like you might ...and restraint method, subcutaneous injections take some practice to get the coordination down. Subcutaneous...theoretically be performed anywhere on the body, somewhere large, flat, and easily accessible (like the back...

...about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication of popularity...section of our plasmid pages is relatively new, so some of our older plasmids may not have an entry in this...Ordering: Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable...store plasmids that need extra stability, such as some of our viral vectors, in NEB Stable cells. Resource...

...updated protocols So that’s great, but what if someone in China improved upon that protocol? How would...frequently it's being updated now, but there are some classic protocols on Protocol Online that you might... As this is a growing market, I am sure I missed some of your favorite apps. If you know of a software...plasmids more successfully. And I’ll continue to add some of our basic protocols to the protocols.io app so...

...and their associated ancestries. While there were some exciting relationships identified, 48% were found...differences in gene expression could explain why some ancestry groups were differentially dependent on...dependencies are undercounted by approximately 20%. In some cases, this may lead researchers to deprioritize...the bias  While their thorough analysis uncovered some concerning biases within widely used CRISPR screens...

...how-to videos on YouTube long before he learned (somewhat successfully) how to text or use Facebook. However...However you choose to communicate your science, some people will love it and others will avoid it like ...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications...convey an entire story or context via still images. Some things cannot be photographed or need text to properly...

...human genome as a map with three coordinates: chromosome, start, and stop. For instance (chr3, 1, 10) ...stretch of DNA at the very beginning of the third chromosome, ten base pairs in length. An emerging family...coordinates alone aren’t very useful. We’ll need something like Google Maps to help us identify and visualize...700 terabytes per gram—and form the X-shaped chromosomes we all know and love. To understand compaction...

...-target DNA editing efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019...transfected cells. C-to-T editing by the APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy...-target DNA editing efficiency of APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction...reporters for single base editing by APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org...

...unmethylated sites in the chromosome. The more stable endonuclease can then cut the chromosome leading to cell ...improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin will...-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine [corrected...

...room names originated from. Let’s take a look at some of the stories behind the names. We also have many...insert specific pieces of DNA into vertebrate chromosomes via a cut and paste mechanism. Scientists reconstructed...couldn’t find the specific origins of the name but some of our Addgenies had an idea: sqh mutant fly ovaries...tour of Addgene’s new conference rooms and learned some fun facts about these genes and the origins of their...

...and hence making it searchable and shareable, but some ELNs provide better support for reproducibility ... use other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to...storage resources remain live. This means that someone who wants to query and attempt to reproduce an ...access restrictions based on permissions, however, in some cases it would be possible to grant those wanting...

... low to nil base editing efficiency at some genomic sites. Some sites may be inaccessible to base editors...editors probably due to the presence of nucleosome or other proteins. You can detect base editing as early...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many...

...and expensive. In some cases, conjugation methods can interfere with binding, and some common antibody ...ELISA, advantages and disadvantages of each, and some considerations when using the method. ELISA basics...sample that is known to contain the target antigen. Some examples could be an endogenous sample of wild type...

...generated through measurement of events. An event is something that passes through the machine’s lasers and gives... read by the machine’s sensors. (Usually this something is a cell, but it could be debris or unbound antibodies...used to identify them, such as “CD123+CD11c- pDCs.” Some cells are defined not by the presence or absence...information FACS plots often contain other data. Some will have numbers by gated populations; this indicates...

...to many complications, including the inability of some institutions to know exactly how many postdocs they...been able to gather data on changes to salary for some of the institutions that are meeting the salary ...length; that postdoc salaries will have to double at some institutions; and that institutions which can more...gradual adjustments towards this new level. Indeed some institutions did this. It doubtless took a lot of...