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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 281 results for: hal.1

Showing: 261 - 280 of 281 results

  1. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated a series of plasmids that...molecules by conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ-3. This system is notable for several... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...your finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified...technologies currently available, you now face the challenge of trying to figure out what system might work...

  6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...off-target effects (Zibitt et al. 2021).    Figure 1: A schematic illustration of CRISPR/Cas9-based approaches...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

  7. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...for your first journal club to begin! Figure 1: Discussing next-generation sequencing at the Addgene...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in the...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math &...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

  12. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...successful and meaningful career in the grants profession: 1. Build on your existing skills & passion  Skills such...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... of low-toxicity RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...

  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...your virus. Let’s start there!     Figure 1: Flowchart showing the process for using pooled lentiviral...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...done is better than perfect. Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community of people...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  19. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn cycle adapted for video...small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer and they...

  20. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...and biotin plasmids at Addgene!     Figure 1: Indirect detection of target markers can be achieved...capacity and speed of cells. (a) With each division half of the dye molecules are lost and the fluorescence...
Showing: 261 - 280 of 281 results