...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...cultures because you are not the first person to face this kind of problem. Educate others when you can...

...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping... 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect to get 500-700 bases...you can purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica...

...or by project, if that works better for you) and place them on an easy to reach shelf. Make sure all notebooks... Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the notebooks... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...find to where the binder was moved. You’ll have peace of mind knowing the information is backed up and...

...to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more... structure to interrogate the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the...perfect guide RNA will do you no good if there is no adjacent PAM to anchor it. However, Cas9 only checks the...HNH domain, ensuring all binding partners are in place before cleavage can occur (Jiang & Doudna, 2017;...

...best place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule...calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking...

...talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity... clones because the bands were all in the wrong place. Except for one. My manager speculated that the ...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

...special words about managing scientists because, let’s face it, we are special. Below are some special characteristics...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...scientists) Are highly mobile and can move to a new workplace if opportunities for learning and personal growth...

...science writing in magazines or newspapers or other places online, and consider how those stories are being...their blogs. 5)   Local science museums are a great place to find opportunities for science outreach. Here...institution; the news or communications office is a good place to start. It’s not unusual to find science writers...Mass Media Fellowship or maybe an internship at a place like Science News. Ask your institutional news office...

...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genes. These data highlight an interesting "arms race" between phages and prokaryotes; the ultimate victor...

...some marketing experience, with little emphasis placed on the marketing or business background of the ...focus on the list of conferences I presented at and placed them under headlines entitled “Presentation Experience...more than necessary for my interview. The simplest place I could think of to start was “Marketing for Dummies...outside of academia, sales and marketing can be great places to start. Depending on what type of career you ...