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  1. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor...

  2. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...yet read the following Top 10 posts of 2014, take some time and see what you’re missing. Let us know if...holiday season and we look forward to seeing what awesome research you and your labs produce in the new year...

  3. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...likely talked to one of us! Today we’re discussing some of our most frequently asked questions related to...payment during checkout. Placing your order online has some benefits. All of the information that is entered...

  4. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...sequencing reactions a week. So I have developed some good habits that I wanted to pass on to you to make...reaction (as opposed to using the all inclusive service some companies offer), you can purify the product either...

  5. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis by PCR obsolete...taking a look at all of our posts as each one has something great to offer, I just need to get back to emailing...

  7. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...and so receives this valuable input from at least some employees. Watch out for "information allies" – ...in on percolating issues in the team.   Here are some other practices to encourage constructive input ...

  8. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...access (OA) movement might be going is to consider some of the places it’s been. So, as 2013 comes to a ...a close, let’s take a few minutes to reflect on some of the most noteworthy developments in open science...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...shared fascinatingly creepy science stories. Here are some of our favorite spooky science stories. Six spooky... blog Read about company culture at Addgene See some of our previous Halloween costume contests ...

  10. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most ...problems in localization that have been encountered by some investigators when using forms of mEos." The ability...

  11. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...Protein purification specialists recommend doing some research, notably determining the subcellular localization...versatile and easy to use, and we hope that it will take some of the stress out of protein purification. For a...

  12. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells using zinc finger nucleases...

  13. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...that stack of papers on your desk, and to tackle some new experiments before the next R01 is due. But ...have added or removed samples. Once you've taken some time to get organized, stop back and let us know...

  14. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...proteins and complexes, without the artifacts sometimes associated with plasmid-based overexpression. ...

  15. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...Addgene’s homepage recently, you probably noticed some big changes to our site (if you haven’t, go check...kept pace with our growth over the last few years. Some of the navigation was buried on the page, and a ...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though DA firing...concentration. To address this, researchers from some labs have developed fluorescent-protein based DA...

  21. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short...helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during...

  22. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...our CRISPR Plasmids and Resources page. Here are some highlights from the past couple months: A new lentiviral...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Our ...

  24. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase...imaging shows only that the bacteria localize to somewhere in the mouse abdomen. arg1 signal, however, can...

  25. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...hydrogen peroxide or ions like calcium and zinc. Some biosensors contain a single fluorescent protein ...information you are looking for. Below we have summarized some of the common fluorescent detection methods and ...

  26. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... processes. Initially, Tanenbaum et al. observed some GFP aggregation, which they reduced by using superfolder...concerns are greater with SunTag. v1 SunTag exhibits some scaffold aggregation (v4 Suntag does not.) It’s...

  28. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses are able...avoids many, though not all, of these defenses. For some applications of genome engineering - such as certain...

  29. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating biological circuits...repository, and I was proud to be responsible for sharing some of our data at this meeting. We weren't quite sure...

  30. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... that aligns with your interests. We've outlined some important factors to consider and summarize helpful... “not be shackled by your own subject - maybe something used in astrophysics can be applied to fungal ...
Showing: 421 - 450 of 745 results