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  1. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral...

  3. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...our synthetic biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment...

  5. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...are so ubiquitous in the lab that it is easy to forget that these enzymes naturally occur in bacteria ...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... would like to thank some of the people and organizations who helped us reach this milestone. Viral service...Research - A Primer for Beginners Resources at Addgene.org Find Virus Protocols Visit Our Viral Vector ...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV ...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...www.ncbi.nlm.nih.gov/books/NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's...

  13. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

  14. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...10):2073. DOI: 10.3390/v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene...

  15. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...favorite content collections is our Help Center, help.addgene.org, where you can easily search articles answering...reach out about writing a guest post (email news@addgene.org.) We are here to support and advance the scientific...

  17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to...

  21. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ...

  22. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...components of biological research that are often forgotten or ignored. If we wish to conduct research that...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations...

  23. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent...

  24. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

  26. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

  27. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...

  28. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...types even when systemically administered in an organism. These features make AAV ideal for human therapies...Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All...

  29. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...Download the Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here...

  30. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...Introduction to Antibodies Additional resources on addgene.org  Antibody GuideRecombinant antibodies available...Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, N., Kim, Y., Lee, ...
Showing: 421 - 450 of 795 results