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We narrowed to 866 results for: FER

Showing: 561 - 600 of 866 results

  1. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways...Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the goal... Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde...

  2. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...large companies, Genzyme and Biogen. They’re very different jobs. Being general counsel at a small start-up...companies. It's one of those "every day is something different" type of jobs, which really suits my personality... Jennifer Tsang...

  3. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...only better for the environment, but most likely safer for researchers, as well. Additional resources...

  4. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ... been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...

  5. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...of the students were interested to see so many different career options in science, with Abigail Anderson...professor. There are amazing opportunities that are offered with a biology major.” After hearing about many...

  6. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9...

  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...antibody. There is one major caveat - a tag may interfere with protein localization and/or function, so ... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

  8. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...

  9. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ... how our articles have helped them understand different topics of science, especially disease related ...terms like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate... receive our science career guide Learn about different careers in science communication Resources on...

  10. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...ultrasound dyes because these dyes “collapse” at different ultrasound frequencies. Read more about ‘Using...communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic reporter...

  11. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ...

  12. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation... Jennifer Tsang...

  13. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes... Jennifer Tsang...

  14. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...then formulate a plan to further develop your transferable skills and reach your career goals.      Although...know your strategies in the comments section!   References 1. Yearly Planning Meetings: Individualized Development... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ...

  15. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...elements. Regulatory DNA function is typically inferred based on correlative biochemical characteristics...Bioengineering from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...Aug 1;289(31):21312-21324. PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information ...

  16. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently...

  17. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX...human cells. Using the structure of CasX at eight different states of assembly from cryo-electron microscopy... Jennifer Tsang...

  18. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...

  19. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...in decreased efficiency of recombination and preferentially leads to gene constructs where the stop cassette...likely generalizable to other systems as well. References: del Valle Rodríguez A, Didiano D, Desplan C...plasmids at Addgene Read our Molecular Biology Reference ...

  20. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...meaningful changes in the lab could make a big difference in our global impact as scientists. Many scientists... single-stream or city recycling services don’t offer plastic recycling, specialized companies can recycle...Sustainable Practices to the Lab: Easy Action Items References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender...

  21. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    .... Northeastern compiles a list of hundreds of different jobs for students to apply to, and after a grueling...marketing and social media co-op. There are a lot of different worries that come along with entering the workforce...of my co-op. I had to do research on loads of different best practices, create an email strategy, and ...

  22. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...per experiment. In the McKay lab we study how differential access to transcriptional regulatory elements...nuclei extraction. They report that this change offers a significant improvement and simplification of...cell or tissue preparation.   All in all, CUT&RUN offers a great alternative to ChIP-seq, especially in ...

  23. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...room for serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments...project's potential. Widening your search creates fertile ground for serendipitous encounters that could ...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...

  24. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...were really leader-specific. How is leadership different from good management? Aren't all career skills...because it emphasizes the importance of these very different roles. At any stage in our careers we may be in...boss) that you are ready to try operating in a different role?  I think the secret sauce can be summed ...

  25. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest...

  27. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...

  28. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and ease of use....moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

  29. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... Jennifer Tsang...

  30. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...)    Science communication, or science writing, refers to the process of communicating science to laypeople...knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers ...field-specific terminology or language. This can refer both to terms that are only used in the field and...

  32. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a... Jennifer Tsang...

  33. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (... using in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

  34. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...enable the conversion of RNA templates to DNA but suffer from several important shortcomings including low... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody...

  35. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...

  36. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...Gel purify your PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

  37. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors. Perhaps most ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

  38. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative word...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...

  39. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and the New Research...sometimes we benefit and sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator...follows an intriguing cast of characters from five different families over several millennia as their paths...

  40. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase reporters. In...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...
Showing: 561 - 600 of 866 results