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We narrowed to 839 results for: LEA

Showing: 561 - 600 of 839 results

  1. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...fantastic model organism for many reasons, not the least of which is ease of transformation. There are many...finding true transformants more difficult. Don’t leave your plants on selective plates for too long: even...recovered from the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won...

  2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...commonly result in a complete loss of function while nuclease-deactivated Cas9 (dCas9) can be tethered to a ...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive ...about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries ...

  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...Third-generation plasmids can be packaged with either system. Please note that the generation used to package the virus...Addgene Blog: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus...

  4. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...subsequently changed fields and my cohort of friends and colleagues had moved on as well, so I knew very few people...people, it was certainly an issue worth addressing. Clear expectations have been established for future participants... after our initial pilot, and look to take some leaps. Binning Singletons is its own organization, working...

  5. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...much trial and error to learn the scripting language, but our path forward was clear: to build one process...assembly algorithms meant that we already had a clear idea of the necessary steps to perform:    ...

  6. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...zebrafish zygote transforms into an organism with a clearly established body plan including head, eyes, and... tracked in whole brains of swimming, actively learning larvae, providing unprecedented views into cognitive...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... variety of expression systems. Keep reading to learn more about the Gateway cloning method and its advantages...conditions, the attL and attR sites can recombine, leading to the excision of the phage from the bacterial...Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony...

  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab...to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember...career center. Alternatively, it might even be a colleague. A mentor does not necessarily need to be someone...

  9. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different...nucleus, if the cell dies from injury, this protein leaks out into the cytoplasm, migrates out of the cell...About Cancer and Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study ...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...CRISPR 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled.... & Liu, D. R. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors....

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens... target cells. Poor tissue culture practices can lead to low titers and poor transductions. Cell lines...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ..., using up valuable resources in the cell thus leading to slowed growth. Additionally, some protein products...well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists ...your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...very high titers (Zingg et al., 2017), which can lead to cells in far away brain regions expressing your...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, ... result in overexpression of your gene and could lead to toxicity or trouble interpreting your results...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close...suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes,...can be used up quickly by abundant transcripts, leading to falsely lowered levels for less abundant transcripts...

  17. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th ...Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences🎵On the...

  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...this series, we’ll highlight one tip that we’ve learned in the lab that will hopefully make your life a...be able to locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab...

  20. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...Guide to Synthetic Biology One of the best ways to learn about a new field is simply to know where to look...have additional resources that might be helpful, please reach out to me by email.  About the author Niko...

  21. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...all of the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

  22. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...’ll discuss the types of programs SITN runs and learn how they think about communicating science. Listen... our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use...

  23. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

  24. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as all that Halloween...yourself on in the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously...

  25. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in 100 µL ...Request webpage, or our announcement blog post to learn more. And our helpful scientific support team is...

  26. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...shared might have you wondering how many plasmids leave Addgene headquarters every day. That would be an...celebration was so much fun! Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids...

  27. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and...idea got off the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption ...

  29. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...happy during their time in grad school. As you'll learn, grad school has its ups and downs for everyone ...Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the ...

  31. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ... we stick to citation counts for a moment, it’s clear that articles in less-prestigious journals have ...citations to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles...

  32. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ..., I drank 3-4 cups of coffee a day, and I had colleagues who were much more wired. Here at #SfN14 there...Addgene in their labs. And it has been exciting to learn about all the amazing research being done with plasmids...

  33. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...generated through your organization in this field. Please also enter the billing address for your organization...department requires a quote in order to generate a PO, learn more about how to create a quote. How do I pay ...

  34. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases HDR efficiency up to 30-...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

  35. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... human cells are avaliable for distribution. To learn more about pooled library screening, read our recent...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some...

  36. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...selectively kills only the bacteria you want it to, leaving all those healthy bugs in the human microbiome ...graduate student in Feng Zhang's lab, in a press release. "In the future, it could allow us to design versions...

  37. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...one is right for you, so if you’re interested in learning more about fluorescent proteins, I invite you ...you to download our Fluorescent Protein eBook and learn much more! ...

  38. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this...

  40. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...number of out of office responses I’ve received). Clearly we have all of our writers to thank for the excellent...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...
Showing: 561 - 600 of 839 results